Overview
Profile across the mouse miRNome
SBI has simplified qPCR profiling of mouse miRNAs by bringing together a comprehensive qPCR array of mouse miRNAs. Our Mouse mmu-miRNome MicroRNA Profiling Kit comes with all the reagents necessary to tag and convert small RNAs into quantifiable cDNA using the sensitive QuantiMir™ technology and a large set of mouse miRNA assays, including major and minor miR forms (709 mature miRNAs).
The kits include assays in two, pre-formatted 384-well assay plates with three endogenous reference miRNA controls on each plate (U1, U6, and RNU43). All microRNA assays are based on miRBase entries.
- Identify miRNA biomarkers and expression pattern signatures
- Rapidly tag and convert all small RNAs into detectable cDNA for qPCR
- Measure as little as picogram amounts of starting total RNA
- Conduct high-throughput screens of cell lines, tissues, and clinical samples
About the included QuantiMir Kit
When you’d like to profile miRNAs with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
- Simple and robust procedure
- Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
- Suitable for high-throughput screening of clinical samples
- Sensitive and accurate
- Versatile—design your own miRNA assays
How It Works
Using the mmu-miRNome MicroRNA Profiling Kit
First, prepare RNA for qPCR using the QuantiMir Kit
Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.
- Tag all small RNAs with a poly-A tail
- Anneal an oligo-dT adaptor
- Reverse transcribe to create first-strand cDNA
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.
Then conduct qPCR assays
Get additional information about the array including miRNA sequences, miRbase accession numbers, and qPCR analysis templates in the Excel document, “Free Analysis Software for Mouse miRNome Ver. 14”
Supporting Data
miRNome Profiler Performance Data
Resources
Citations
- Olivieri, F, et al. (2016) Circulating miRNAs and miRNA shuttles as biomarkers: Perspective trajectories of healthy and unhealthy aging. Mech. Ageing Dev..2016 Dec 13;. PM ID:27986629
- Khamaisi, M, et al. (2016) PKCδ inhibition normalizes the wound-healing capacity of diabetic human fibroblasts. J. Clin. Invest..2016 Mar 1; 126(3):837-53. PM ID:26808499
- Pandey, S, et al. (2015) The RNA Stem-Loop to G-Quadruplex Equilibrium Controls Mature MicroRNA Production inside the Cell. Biochemistry.2015 Dec 8; 54(48):7067-78. PM ID:26554903
- Meiler, S, et al. (2015) MicroRNA 302a is a novel modulator of cholesterol homeostasis and atherosclerosis. Arterioscler. Thromb. Vasc. Biol..2015 Feb 1; 35(2):323-31. PM ID:25524771
- 岡本行広, , et al. (2015) マイクロ抽出場によるヒト腫瘍細胞由来のマイクロ RNA 抽出法. 分析化学.2015 Jan 31; 64(1):9-13. Link:分析化学
- Kumarswamy, R, et al. (2012) SERCA2a gene therapy restores microRNA-1 expression in heart failure via an Akt/FoxO3A-dependent pathway. Eur. Heart J..2012 May 1; 33(9):1067-75. PM ID:22362515
- Alpini, G, et al. (2011) Regulation of placenta growth factor by microRNA-125b in hepatocellular cancer. J. Hepatol..2011 Dec 1; 55(6):1339-45. PM ID:21703189
- Crother, TR, et al. (2011) Chlamydia pneumoniae infection induced allergic airway sensitization is controlled by regulatory T-cells and plasmacytoid dendritic cells. PLoS ONE.2011 Jun 22; 6(6):e20784. PM ID:21695198
- Stellato, C, et al. (2011) Glucocorticoid (GC) Modulation of Global miRNA Profile in Human Airway Epithelial Cells. Journal of Allergy and Clinical Immunology.2011 Feb 1; 127(2S):AB64. Link:Journal of Allergy and Clinical Immunology
- Karim, Z, et al. (2011) Signaling Mechanism of Tolerance following Repetitive Stimulation with a Single Allergen and its Reversal by Stimulation with Multiple Allergens. Journal of Allergy and Clinical Immunology.;:AB64. Link:Journal of Allergy and Clinical Immunology
SystemBiosciences,简称SBI,美国加利福尼亚湾区新成立的生技公司,致力于独特,创新生物技术之开发,以研发利于基因及蛋白质功能鉴定,研究之崭新方法和工具为宗旨。
美国SBI代理SystemBiosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。现阶段研发重心为RNA干扰(RNAi)研究之相关工具。
现阶段研发重心为RNA干扰(RNAi)的研究之相关工具。系统Biosciences公司(SBI)致力于开发独特,革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具.SBI是专业的慢病毒产品公司,提供基于慢病毒的所有相关产品,质粒,试剂盒及相关配套试剂和慢病毒扩展产品,如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。System Biosciences继续创造新的独特产品以及改善我们完善的产品线。我们对提供领先技术的承诺与我们所有研究试剂和研究项目服务的高质量制造和质量控制相匹配。 2009年要寻找的东西:以下是即将推出的新产品。新型miRZips™:基于慢病毒载体的新型技术可永久敲低MicroRNA。双标记shRNA表达载体:SBI将发布新的功能强大的shRNA表达慢病毒载体pGreenPuro™,以便为稳定的RNAi实验选择稳定转导的细胞的GFP和Puro标记。甾醇反应pGreenFire™慢病毒报告子:基于慢病毒载体的转录报告子,用于监测与固醇感应转录因子相关的转录网络活性-心血管疾病途径的关键。诱导型表达慢载体:新的构建体将允许CDNA,shRNA和microRNA的“按需”表达。2007–2008年的新产品新的miR-SNaRE:MicroRNA小型非编码RNA富集系统,该系统使用带有表位标签的microRNA加工因子来提取蛋白质及其相关RNA。识别驱动RISC复合体的信使RNA。新的GeneNet™聚焦的shRNA库:这些聚焦的shRNA库编码一组针对所有与人类激酶,磷酸酶或细胞凋亡相关基因有关的特定功能或类别基因设计的shRNA集合。这些文库可进行有针对性的高通量RNAi筛选。新的miRNomeMicroRNA分析仪:qPCR阵列在预先格式化的板中包括microRNA分析,可用于人类的完全互补或小鼠单个microRNA的完全互补,每块板上带有三个内源参考RNA对照。所有基于SangermiRBasemicroRNA数据库的microRNA分析均已注册。新的Lenti-miR™MicroRNA前体克隆:在基于HIV的慢病毒载体中可获得更多的microRNA前体集合。超过580个单独的microRNA前体克隆可用阵列形式或合并的慢病毒形式用于HT筛选。新的基于慢病毒的干细胞报道者:SBI越来越多的慢病毒载体已被开发为将基因构建体在体内外几乎传递给任何细胞类型的最有效方法。SBI已将我们的慢病毒构建体系列扩展为干细胞报道分子。使用连接到GFP报告基因的细胞和途径特异性启动子,轻松创建转基因细胞系以监测细胞分化。QuantiMir™RT试剂盒:这项流行的新技术可通过一次cDNA合成同时进行实时qPCR定量分析数百种microRNA。设计您自己的microRNA测定法以进行创新性实验。癌症microRNAqPCR分析小组(OncoMir系列):预格式化的microRNA分析小组,用于评估95种已知与癌症有关的microRNA。干细胞microRNA分析小组:介绍了95种参与干细胞分化的microRNA,可同时监测干细胞的自我维持,造血途径和神经分化。SBI完善的产品线FullSpectrum™完整信使RNA扩增试剂盒:利用针对mRNA最常见基序的mRNA特异性引物提供完整的mRNA转录物(5"和3"末端)的无偏见,完整代表。GeneNet™siRNA库:全基因组,即用型,预包装的慢病毒库为筛选与生物学反应相关的基因功能提供了令人兴奋的机会。干扰素反应检测试剂盒:区分真正的RNA干扰和压力相关的细胞反应。PathNet™转录报告基因慢病毒载体:一种独特的方法,可创建各种稳定的报告基因细胞系,用于信号通路的研究。我们感谢您过去的支持,并期待为您提供最佳的新试剂,技术和服务,以加速您成功的研究目标。
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德国MoBiTec公司于1987年由Stephan Diekmann 教授创立,由于和Max-Planck协会(德国处于领先地位的研究中心)有着密切的关系,这使得本公司与很多具有创造性的科学家有着亲密的合作。另外,MoBiTec 拥有来自欧洲和北美的研究人员,包括法国的Pasteur 研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计,而留出更多更宝贵的时间用于实际的研究。
产品包括: 双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系,噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质,Lamda PS基因文库,用于克隆的多功能DNA载体,靶基因的表达与分析,重组蛋白质高效表达和纯化试剂盒,细胞转染试剂和细胞培养工具,各种固体和溶解状态的酶,基因组学和蛋白组学研究产品(PCR,核酸,蛋白质纯化分析),各种抗体、细胞因子、生长因子、重组蛋白质,高级荧光试剂和试剂盒(蛋白质标记试剂盒,标记抗体,细胞染色试剂,钙指示器),亲和层析仪,凝胶分析工具——凝胶柱和凝胶电泳附件。
Bacillus megaterium Expression System
Bacillus subtilis Expression Vectors
pBacTag Tagging Vectors
pPICHOLI Shuttle Vector System
Broad-Host-Range Vectors pBBR122 & pBHR1
Fusion Protein Cloning System PheBo
Fusion Protein Cloning System pAX
BRP Plasmid and Competent BRP Cells
pPEPTIDE Cloning Vector
Promoter-Trap Vector pBBR RESO
pORF-CLONE Vector System
Exontrap Cloning Vector
PCR Cloning Vector p3T
Suicide Vector pCorrectClone
ssDNA-Production and Expression in pMEX
His-tag Cloning Vector pEG-His1
Multiple Cloning Site Vector pMCS5
Your partner for unique Two- and One-Hybrid-Systems, Phagemid Display, Genomic Libraries, DNA Vector Systems, Cell Biology and Antibodies, DNA and Protein Tools, Fluorescence Technology, Affinity Chromatography, and Lab Supplies
about MoBiTec
History
nnovative Tools for Molecular & Cell Biology
MoBiTec GmbH is a privately held company that was founded by Prof. Dr. Stephan Diekmann in 1987. The close relation to the Max-Planck Society as well as the location in Goettingen, one of Germany’s leading research centers, facilitated close cooperation with some of Germany’s most inventive scientists. In addition, MoBiTec has always been operating extensively with scientists all over Europe and North America, including those from the Pasteur Institute in France and Vrije University in the Netherlands. By commercializing the brilliant ideas of many researchers, MoBiTec is able to offer a unique range of high quality products with simplified protocols making lab life easier and leaving more time for actual research.
Products include e. g. two- & one hybrid systems for in vivo detection of protein-protein or DNA-protein interactions, a phagemid display system for screening specific peptide ligands and selecting novel proteins with specific binding properties, convenient Lambda PS genomic libraries; versatile DNA vectors for cloning, expressing and analyzing target genes, kits for efficient expression and purification of recombinant proteins, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, many products for genomics and proteomics research (e. g. for PCR, nucleic acid & protein purification and analysis) , numerous antibodies, cytokines, growth factors and recombinant proteins, superior fluorescence reagents and kits (e. g. protein labeling kits, labeled antibodies, cell staining reagents, calcium indicators), affinity chromatography products, gel analysis tools as well as general laboratory equipment such as lab columns and gel electrophoresis accessories.
In parallel to its own product lines, MoBiTec distributes products from several international companies in Germany including fluorescent probes and research chemicals (Molecular Probes, AnaSpec, Teflabs), quantum dots (Evident Technologies), a variety of DNA purification kits and DNA polymerases (Edge Biosystems, TaKaRa), diagnostic tools (Biomerica, Cytocell), gel elecrophoresis accessories (Amresco), glycobiology products (Dextra Laboratories), high-quality biochip products (GenTel), cell transfection reagents (Mirus) as well as antibodies, proteins, substrates, fine chemicals and kits for molecular and cell biology - in particular virology, immunology, neurobiology, apoptosis, signal transduction, cell proliferation, cytotoxicity and cancer research (MBL, A.G. Scientific, TaKaRa, Amresco, ViroGen, AnaSpec).
MoBiTec offers a total service, beginning with the clear and full documentation of products extending to individual advice and technical service for customers. MoBiTec products are distributed worldwide - in Germany from our home office, in other countries by distributors. Moreover, MoBiTec is continuously looking for new products and marketable technologies for licensing and is therefore strongly interested in scientific collaborations in order to develop innovative product ideas.
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如果你需要购买 药品对照品、标准品,可以到中国药品生物制品检定所订购,也可以查询中国标准物质网订购。
蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!
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NBS 是 Bio Basic Inc 的一部分,我们能够在英国提供全方位的 Bio Basic 定制测序服务。Bio Basic 是世界上最大的专业基因制造商之一。15 年来,他们作为一家无声的 OEM,为全世界的研究人员合成了数百万个碱基对的基因。
现在,我们很高兴能够直接以其自己的名义提供 Bio Basic 著名的基因合成服务。受益于其寡核苷酸库和 DNA 测序设施,我们以业内最实惠的价格提供基因合成。
Bio Basic 提供各种基因服务以满足任何实验室的需求。从大型项目到小型定制项目,我们可以帮助您选择能为您带来最大利益的服务。
我们的基因合成过程
1
寡核苷酸合成
具有相似解链温度 (Tm) 的多个长寡核苷酸(长度约 50-60 个碱基)根据所需的最终基因序列化学合成为片段。寡核苷酸被设计为通过重叠序列相互组装。
2
基因组装
使用 PCR 技术,寡核苷酸片段被组装成长达 1kb 的双链 DNA 块。然后使用 PCR 再次组装和扩增这些块,以创建一个大的双链 DNA 构建体。
3
克隆
合成的 DNA 被插入到特定的载体中。然后分离并扩增载体。除非另有说明,使用的默认载体是 pUC57-Amp 载体。
单击此处查看我们的矢量列表。
4
选择菌落
菌落生长,然后选择合适的含有目的合成基因的菌落。
5
DNA测序
所有最终基因都通过 DNA 测序验证,只有那些具有正确序列的基因才会被选中。这确保合成的基因符合项目开始时计划的规格。在这里,使用 Sanger 测序是因为它的读取长度长,运行时间短。
6
冻干
应用进一步的 QC 措施并将基因冻干到微量离心管中。该基因现已准备好用于下游应用。
芯片上的基因
通过引入下一代基因合成方法 - CHIP 技术,预计未来几年基因合成的价格将显着降低。因此,拥有一个能够适应这个不断变化的市场的合作伙伴将是一笔巨大的财富。
BioBasic 致力于通过对并行芯片基因合成技术等新技术的战略投资来保持市场竞争力。
拥有 BioBasic 作为您的合作伙伴将使您能够继续满足并超越您的期望和需求,而不会因在这个不断变化的市场中更换合作伙伴而带来的压力和并发症越来越大。
链接:http://www.nsjbio.com/
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NSJ BioreagentsX品牌 历史介绍:苏州蚂蚁淘生物科技有限公司,作为一家生命科学领域的高科技生物公司,目前代理销售二十多家欧美著名生物技术公司的产品,覆盖了免疫学、细胞生物学、分子生物学、药物筛选、生物制药、食品检测、疫苗生产等领域。
我问了两个实验员,一个说可以,一个说不行。
说不行的理由:1:会把RNA和DNA打断。2:超声破碎过程中会产热,会降解RNA。
请问有人用过破碎仪提取总RNA或基因组DNA吗?如果能,请问电压调到多少?还有哪些要求。
另外关于破碎仪消毒的问题,能用酒精棉球擦拭,再用无菌棉片檫干,再放入trizol中超声破碎吗?还有没有更好的方法?
谢谢!
LKT Labs
LKT LabsX品牌 历史介绍:苏州蚂蚁淘生物科技有限公司,作为一家生命科学领域的高科技生物公司,目前代理销售二十多家欧美著名生物技术公司的产品,覆盖了免疫学、细胞生物学、分子生物学、药物筛选、生物制药、食品检测、疫苗生产等领域。
苏州蚂蚁淘生物科技有限公司成立于2017年,是一家专业提供实验室整体采购方案的专业公司,为广大采购和实验室解决了大量问题,几年来已经成为所有合作伙伴最优秀的合作伙伴,我们一直秉承信誉第一的原则,坚持不卖假货,哪怕利润损失也要为客户提供最好的产品
我们已经和国外500多个品牌建立了贸易往来,并在国际上享有相当知名度,现已经成为国外近100多个品牌中国指定代理,并成为其中国最优秀的代理商
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