Product Name
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Part No.
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Unit Size
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Concentration
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Notes
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NEW PRODUCTS:
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E. coli DNA Ligase
|
L6090L
|
2,500
|
10,000 U/ml
|
Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG
|
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T4 RNA Ligase 2
|
L6080L
|
4,500
|
30,000 U/ml
|
Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures
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T4 RNA Ligase 2 Truncated
|
L6070L
|
500
|
5,000 U/ml
|
Requires a preadenylated 5′ phosphate containing DNA or RNA to ligate to 3′ hydroxyl of RNA
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Omni Klentaq®
|
P7500-HC-L
|
8,400
|
42,000 U/ml
|
Engineered Taq Polymerase mutant which enables PCR direct from blood, soil, water and food samples. Increased tolerance to fluorescent intercalating dyes
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(High Concentration)
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Omni Klentaq®
|
P7500-LC-L
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8,400
|
4,200 U/ml
|
Lower concentration – 1 microliter per reaction
|
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(Low Concentration)
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Phoenix Hot Start Taq DNA Polymerase
|
P7590L
|
500
|
5,000 U/ml
|
Antibody based Hot Start Taq DNA polymerase delivers robust PCR performance with exceptional pre-PCR cycling room temperature stability
|
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VeraSeq™ 2.0 High-Fidelity DNA Polymerase
|
P7511L
|
500
|
2,000 U/ml
|
Industry-leading speed, fidelity and robustness delivered via an engineered PCR polymerase
|
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VeraSeq™ ULtra DNA Polymerase
|
P7520L
|
500
|
2,000 U/ml
|
High fidelity, speed, and robustness in PCR delivered by an engineered, thermostable DNA polymerase that tolerates uracil templates, nucleotides and primers
|
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RecA
|
Y9260L
|
1.5 mg
|
2.0 mg/ml
|
Promotes strand exchange of single-stranded DNA fragments with DNA duplex substrates
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Thermolabile Phosphatase
|
Y9210L
|
5,500
|
5,000 U/ml
|
Removes 5′ and 3′-phosphate groups from DNA
|
|
Thermolabile UDG
|
G5020L
|
500
|
1,000 U/ml
|
Removes uracil from DNA, leaving AP site
|
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Poly A Polymerase
|
P7460L
|
1,000
|
5,000 U/ml
|
Catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA which is useful for making polyA tailed RNA
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Ultra Pure Ligases:
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Bitmap E. coli DNA Ligase
|
L6090L
|
2,500
|
10,000 U/ml
|
Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG
|
|
T3 DNA Ligase
|
L6010L
|
900,000
|
3,000,000 U/ml
|
Works in high ionic strength – to 1.0 M NaCl
|
|
T4 DNA Ligase
|
L6030-LC-L
|
150,000
|
120,000 U/ml
|
Low concentration overnight ligation format
|
|
T4 DNA Ligase (Rapid)
|
L6030-HC-L
|
240,000
|
600,000 U/ml
|
“Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008)
|
|
Bitmap T4 RNA Ligase 1
|
L6050L
|
10,000
|
20,000 U/ml
|
Single-stranded RNA ligase. Also joins single-stranded DNA molecules
|
|
T4 RNA Ligase 2
|
L6080L
|
4,500
|
30,000 U/ml
|
Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures
|
|
Bitmap T4 RNA Ligase 2 Truncated
|
L6070L
|
500
|
5,000 U/ml
|
Requires a preadenylated 5′ phosphate containing DNA or RNA to ligate to 3′ hydroxyl of RNA
|
|
T7 DNA Ligase
|
L6020L
|
900,000
|
3,000,000 U/ml
|
1000 fold higher activity on sticky ends than blunt ends
|
|
Taq DNA Ligase
|
L6060L
|
20,000
|
40,000 U/ml
|
Thermostable DNA ligase which efficiently seals nicks and discriminates against mismatch ligation
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Ultra Pure Polymerases:
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DNA Polymerase I
|
P7050L
|
5,000
|
10,000 U/ml
|
DNA polymerase with 5′→ 3′ synthesis,
|
|
5′→ 3′ and 3′→ 5′ exonuclease activities
|
|
Klenow (3′→ 5′ exo-)
|
P7010-HC-L
|
10,000
|
50,000 U/ml
|
Proven choice for DNA labeling, A-tailing
|
|
Klenow (3′→ 5′ exo-)
|
P7010-LC-L
|
10,000
|
5,000 U/ml
|
Proven choice for DNA labeling, A-tailing
|
|
Klenow Fragment
|
P7060L
|
2,500
|
5,000 U/ml
|
DNA polymerase useful for end-filling prior to blunt-end ligation
|
|
Mako DNA Polymerase
|
P7090L
|
3,000
|
30,000 U/ml
|
Exonuclease-deficient polymerase, lacks strand displacement activity
|
|
Manta 1.0 DNA Polymerase
|
P7140-HC-L
|
100,000
|
400,000 U/ml
|
Thermostable Bacillus DNA Polymerase, strong strand displacement
|
|
(High Concentration)
|
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Manta 1.0 DNA Polymerase
|
P7140-LC-L
|
100,000
|
40,000 U/ml
|
Thermostable Bacillus DNA Polymerase, strong strand displacement
|
|
(Low Concentration)
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|
Bitmap M MuLV Reverse Transcriptase
|
P7040L
|
100,000
|
200,000 U/ml
|
Useful polymerase for first-strand DNA synthesis
|
|
Omni Klentaq®
|
P7500-HC-L
|
8,400
|
42,000 U/ml
|
Engineered Taq Polymerase mutant which enables PCR direct from blood, soil, water and food samples. Increased tolerance to fluorescent intercalating dyes
|
|
Bitmap (High Concentration)
|
|
Omni Klentaq®
|
P7500-LC-L
|
8,400
|
4,200 U/ml
|
Lower concentration – 1 microliter per reaction
|