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GenWay/Human Alpha-1-Antitrypsin ELISA Quantitation Kit/GWB-1F2730/10x96 Wells
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4000-520-616
Description:
| Species | Human |
|---|---|
| SampleType | Serumorplasma.ForthequantitativedeterminationofhumanAlpha1-AntitrypsinlevelsinserumorotherBIOLOGicalsamples.Blood,ConnectiveTissueDiseases,LungDiseases,Liver,PathologicProcesses. |
| ProductName | Humanalpha1-Antitrypsin. |
| SampleType | Serumorplasma. |
| #ofSamples | 10X96wells. |
| StorageandStABIlity | -20°C. |
| RangeofDetection | 1.4-90ng/ml |
| Shelflife | oneyearfromdateofreceipt. |
| AssayCondition | ThekitperformancehasbeenoptimizedfortheprotocolandmaterialslistedbelowusingstandarddilutionsofhumanAlpha-1-Antitrypsininthe1.4-90ng/mlrange.Theoperatormustdetermineappropriatedilutionsofreagentsforalternativeassayconditions.ELISAassayreactivityissensitivetovariationsinoperator,pipettingandwashingtechniques,incubationtime,temperature,compositionofreagents,andotherexperimentalvariables.Assayoptimizationmayberequiredtogeneratethestandardcurveandfitthesamplesinthespecifieddetectionrange. |
| CountryofOrigin | UnitedStatesofAmerica. |
| AssayUse | ForResearchUseOnly(RUO).Notfordiagnosticortherapeuticuseinhumansoranimals.Notforanimalorhumanconsumption. ProtocolsfromPublicationsCitation:1:PottGB,ChanED,DinarelloCA,ShapiroL.Alpha-1-antitrypsinisanendogenousinhibitorofproinflammatorycytokineproductioninwholeblood.JLeukocBiol.2009May;85(5):886-95.Epub2009Feb5.PubMedPMID:19197072;PubMedCentralPMCID:PMC2669404. |
| ExperimentName | ELISA. |
| ExperimentBackground |
|
| ExperimentSteps | DetermineAATconcentrationsfromAAT-deficientandhealthyvolunteersinheparanizedplasmausingahumanAATquantitativeELISA. |
| Function | Inhibitorofserineproteases.Itsprimarytargetiselastase,butitalsohasamoderateaffinityforplasminandthrombin.Inhibitstrypsin,chymotrypsinandplasminogenactivator.Theaberrantforminhibitsinsulin-inducedNOsynthesisinplatelets,decreasescoagulationtimeandhasproteolyticactivityagainstinsulinandplasmin. |
| SubcellularLocation | Secreted. |
| TissueSpecificity | Plasma. |
| Domain | Thereactivecenterloop(RCL)extendsoutfromthebodyoftheproteinanddirectsbindingtothetargetprotease.TheproteasecleavestheserpinatthereactivesitewithintheRCL,establishingacovalentlinkagebetweenthecarboxylgroupoftheserpinreactivesiteandtheserinehydroxyloftheprotease.Theresultinginactiveserpin-proteasecomplexishighlystable. |
| Ptm |
|
| Polymorphism | ThesequenceshownisthatoftheM1VallelewhichisthemostcommonformofPI(44to49%).Otherfrequentallelesare:M1A20to23%;M210to11%;M314to19%. |
| Disease |
|
| Miscellaneous | Theaberrantformisfoundintheplasmaofchronicsmokers,andpersistsaftersmokingisceased.Itcanstillbefoundtenyearsaftersmokinghasceased. |
| Similarity | Belongstotheserpinfamily. |
| ProductProtocol | Toseethisproductusedinapeerreviewedjournal,clickthefollowinglinkα-1-Antitrypsinisanendogenousinhibitorofproinflammatorycytokineproductioninwholeblood. |
AdditionalInformation:
| Name | HumanAlpha-1-AntitrypsinELISAQuantitationKit |
|---|---|
| RelatedProductNames | Alpha-1proteaseinhibitor,Alpha-1-antiproteinase,Humanalpha1-AntitrypsinELISAKit;Alpha-1proteaseinhibitor,Alpha-1-antiproteinase,AAT,PIHumanalpha1Antitrypsin,SERPINA1,PI,AATELISA,A1AT |
| ShortDescription | Humanalpha1-Antitrypsin,AAT,AATELISA |
| NCBIAccNumber | NP_000286.3 |
| MolecularWeight | 46737 |
| SwissProtNumber | P01009 |
| IntendedUse | ResearchUseOnly |

