Description:

Product Name: HSV-1 IgG ELISA (Mouse/Rat)

Sample Type: Serum/Plasma

# of Samples: 1x96 well

Intended Use: The GenWay, Inc. Mouse HSV-1 IgG ELISA test system is an enzyme linked immunosorbent assay (ELISA) is used for the detection of IgG class antibodies to HSV-1 in mouse

Principle of the test: Diluted serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Summary and Explanation of Test: Storage and Stability: 1. Store the kit at 2-8 C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.

Storage and Stability: 1. Store the kit at 2-8 C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.

Limitation of the Test: 1. The test results obtained using this kit can not discriminate between HSV-1 and HSV-2 infection due to high cross reactivity between the two viruses. The results serve only as an aid to diagnosis and should be interpreted in relation to the patient's history, physical findings and other diagnostic procedures.
2. Lipemic or hemolyzed samples may cause erroneous results.

Sensitivity and Specificity: 336 patient sera were tested by this HSV-1 IgG ELISA and a reference ELISA method. 270 sera were positive and 56 were negative by both methods (97% agreement). The results are summarized below:

Product Name: HSV-1 IgG ELISA (Mouse/Rat)

Additional Information:

Name	HSV-1 IgG ELISA (Mouse/Rat)
Related Product Names	HSV-1 IgG ELISA (Mouse/Rat)
Molecular Weight	0.5
Intended Use	Research Use Only