High Sensitive ELISA Kit for Interleukin 6 (IL6)
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
- Product No.HEA079Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.49pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 557 For more details, please contact local distributors!US$ 796 For more details, please contact local distributors!US$ 3582 For more details, please contact local distributors!US$ 6766 For more details, please contact local distributors!US$ 55720 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 6 (IL6).No significant cross-reactivity or interference between High Sensitive Interleukin 6 (IL6) and analogues was observed.
Recovery of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
Matrices listed below were spiked with certain level of recombinant High Sensitive Interleukin 6 (IL6) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Interleukin 6 (IL6) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-102 | 98 |
| EDTA plasma(n=5) | 87-99 | 95 |
| heparin plasma(n=5) | 91-98 | 94 |
Precision of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Interleukin 6 (IL6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 90-104% | 96-104% | 91-98% |
| EDTA plasma(n=5) | 95-102% | 79-90% | 79-102% | 85-101% |
| heparin plasma(n=5) | 82-97% | 90-102% | 79-103% | 80-102% |
Stability of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the High Sensitive ELISA Kit for Interleukin 6 (IL6)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Bos taurus; Bovine (Cattle) | Applications (RESEARCH USE ONLY!) |
| RPA079Bo01 | Recombinant Interleukin 6 (IL6) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA079Bo01 | Polyclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC; IP. |
| MAA079Bo21 | Monoclonal Antibody to Interleukin 6 (IL6) | WB; IHC; ICC; IP. |
| SEA079Bo | ELISA Kit for Interleukin 6 (IL6) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA079Bo | High Sensitive ELISA Kit for Interleukin 6 (IL6) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA079Bo | CLIA Kit for Interleukin 6 (IL6) | Chemiluminescent immunoassay for Antigen Detection. |
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
