Instant ELISA Kit for Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- Product No.IEA033Gu
- Organism SpeciesCavia (Guinea pig ) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length1h, 10min
- Detection Rangen/a
- Sensitivityn/a
- Sample Typen/a
- Downloadn/a
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 756 For more details, please contact local distributors!US$ 1080 For more details, please contact local distributors!US$ 4860 For more details, please contact local distributors!US$ 9180 For more details, please contact local distributors!US$ 75600 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Instant ELISA Kit for Interferon Alpha (IFNa)
This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa).No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed.
Recovery of the Instant ELISA Kit for Interferon Alpha (IFNa)
Matrices listed below were spiked with certain level of recombinant Instant Interferon Alpha (IFNa) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Interferon Alpha (IFNa) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-90 | 84 |
| EDTA plasma(n=5) | 81-94 | 87 |
| heparin plasma(n=5) | 94-101 | 98 |
Precision of the Instant ELISA Kit for Interferon Alpha (IFNa)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Instant ELISA Kit for Interferon Alpha (IFNa)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Interferon Alpha (IFNa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-95% | 78-90% | 95-104% | 87-101% |
| EDTA plasma(n=5) | 84-97% | 78-96% | 91-99% | 92-104% |
| heparin plasma(n=5) | 79-88% | 79-93% | 78-91% | 99-105% |
Stability of the Instant ELISA Kit for Interferon Alpha (IFNa)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Instant ELISA Kit for Interferon Alpha (IFNa)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the Instant ELISA Kit for Interferon Alpha (IFNa)
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interferon Alpha (IFNa). Standards or samples and HRP-labeled detection antibody specific to Instant Interferon Alpha (IFNa) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Interferon Alpha (IFNa), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Cavia (Guinea pig ) | Applications (RESEARCH USE ONLY!) |
| RPA033Gu02 | Recombinant Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA033Gu01 | Recombinant Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA033Gu02 | Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| PAA033Gu01 | Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| MAA033Gu21 | Monoclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| IEA033Gu | Instant ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA033Gu | ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
