- Extract high quality & purity total RNA including miRNA
- No phenol step required; isolate all RNA in one fraction
- Genomic DNA Removal Column for efficient elimination of gDNA
- Bind & elute all RNA irrespective of size or GC content, without bias
- Efficiently extract small RNA irrespective of GC content
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Convenient & fast spin column format
- Isolate from a wide variety of specimens
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Total RNA Purification Plus Kit
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
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采用抗-HBe抗体包被反应板,加入校准品及被测样本,同时加入定量HBeAg中和抗原,经过振荡孵育,洗板后再加入铕标记的抗-HBe,若标本中抗-HBe浓度高,HBeAg将被大量中和,使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液(β-NTA)将标记在抗体上的Eu3+解离到溶液中,Eu3+和增强液中的有效成分形成高荧光强度的螯合物,荧光强度和样本中的抗-HBe浓度成反比。
GM实验:试剂说明书上要求20分钟显色,10分钟的时候看一下,差不多就可以加终止液,但是我的标曲显色特别快,2分钟最低浓度蓝色就就深了,此时样本颜色还很浅很浅,我只能一块儿加终止液。所以,标曲显色过快,可能是什么原因?
(ps:第一列是标曲,后面是样本)



