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Norgen Biotek /Animal Tissue RNA Purification Kit (Cat. 25700)/50preps/25700
免费咨询热线
4000-520-616
- Extract high quality & purity total RNA including miRNA
- Isolate from a wide variety of animal tissues
- No phenol step required - isolate all RNA in one fraction
- Compatible with tissues stored in RNAlater® or Trizol®
- Convenient & fast spin column format
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors kits, Norgen"s Animal Tissue RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA without the use of phenol. Total RNA was isolated from 25 mg samples of rat muscle using Norgens Animal Tissue RNA Purification Kit and a leading competitor"s kit. Five microliters of the 50 µL RNA eluted from each kit was then resolved on a 1.2% formaldehyde-agarose gel. As it can be seen, only Norgens kit was able to isolate the full diversity of RNA species, including all the small RNA species]">
Animal Tissue RNA Purification Kit Figure 1
Figure 2. High Quality RNA Purification from a Diversity of Tissues.Norgens Animal Tissue RNA Purification Kit isolates high quality RNA from a diversity of tissues including both tissues with high RNA content and fibrous tissues. Total RNA was successfully isolated from various tissue samples including 10 mg of spleen (Panel C), 10 mg of kidney (Panel D) and 25 mg of fiber-rich muscle (Panel B) using Norgens Animal Tissue RNA Purification Kit and a leading competitor"s kit (Panel A). All RNA samples were eluted in a 50 µL volume. One microliter of each of the purified RNA samples was then analyzed using an Agilent BioAnalyzeron an RNA Nano 6000 Chip. All RNA samples resolved well in the Bioanalyzer suggesting high RNA quality. Moreover, small RNA could be observed in all the RNA samples isolated using Norgens kit (Panels B-D, circled), whereas no small RNA was present in the samples isolated using a competitors kit (Panel A).]">
Animal Tissue RNA Purification Kit Figure 2
Figure 3. Total RNA with High Yield of microRNA Species. Unlike most competitor kits that exclude RNA species of size < 200 nt, Norgens Animal Tissue RNA Purification Kit isolates the complete size range of total RNA including small RNAs. Total RNA was isolated from 25 mg of a rat muscle sample using Norgens Animal Tissue RNA Purification Kit and a leading competitors kit as shown in Figure 1. Two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the rat miR-21 (Panel A) and the beta-actin transcripts (Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA. As it can be seen, Norgens kit isolated much higher yields of microRNA, as indicated by the lower Ctvalue of the blue line in Panel A. The kits isolated similar yields of mRNA as seen by the similar Ctvalues in Panel B. NTC corresponds to no-template control.]">
Animal Tissue RNA Purification Kit Figure 3
Figure 4. Compatibility with Tissues Stored in RNAlater®. Norgens Animal Tissue RNA Purification Kit can isolate total RNA from both flash frozen tissues and tissues stored in RNA preservation media such as RNAlater®. Total RNA wasisolated from 10 mg of a hamster kidney sample, either flash frozen or stored in RNAlater®, using Norgens Animal Tissue RNA Purification Kit and a leading competitors kit. Three microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT. One microliter of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the hamster miR-21 (Panel A) and the beta-actin transcripts (Panel B), respectively. In both graphs the blue lines correspond to Norgen isolated-RNA and the red lines correspond to competitor-isolated RNA, both from RNAlater®-preserved samples. The green lines correspond to Norgen isolated-RNA from flash frozen samples. As it can be seen, Norgens kit isolated similar amounts of both mRNA and microRNA from either frozen or RNAlater®-preserved samples, as shown by the similar Ct values of the blue and green lines. In addition, Norgens kit isolated much higher yields of microRNA than the competitors kit, as indicated by the lower Ctvalue of the blue and green lines in Panel A. The kits isolated similar yields of mRNA as seen by the similar Ctvalues in Panel B. NTC corresponds to no-template control.]">
Animal Tissue RNA Purification Kit Figure 4
Animal Tissue RNA Purification Kit
This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
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蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠Endothelin-1单抗包被于酶标板上，标准品和样品中的Endothelin-1与单抗结合，加入生物素化的抗小鼠Endothelin-1，形成免疫复合物连接在板上，辣根过氧化物酶 查看更多
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠eNOS单抗包被于酶标板上，标准品和样品中的eNOS与单抗结合，加入生物素化的抗小鼠eNOS，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin与 查看更多
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠G-CSF单抗包被于酶标板上，标准品和样品中的G-CSF与单抗结合，加入生物素化的抗小鼠G-CSF，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin 查看更多
>
2018-12-26
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠dsDNA单抗包被于酶标板上，标准品和样品中的dsDNA与单抗结合，加入生物素化的抗小鼠dsDNA，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin 查看更多
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2018-12-26
st1:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable{mso-style-name:普通表格;mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-no 查看更多
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠Fibrinogen单抗包被于酶标板上，标准品和样品中的Fibrinogen与单抗结合，加入生物素化的抗小鼠Fibrinogen，形成免疫复合物连接在板上，辣根过氧化物酶标记 查看更多
>
2021-08-22
v:* {behavior:url(#default#VML);}o:* {behavior:url(#default#VML);}w:* {behavior:url(#default#VML);}.shape {behavior:url(#default#VML);}st1:*{behavior:url(#ieoo 查看更多
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2021-08-16
v:* {behavior:url(#default#VML);}o:* {behavior:url(#default#VML);}w:* {behavior:url(#default#VML);}.shape {behavior:url(#default#VML);}st1:*{behavior:url(#ieoo 查看更多
>
采用双抗体夹心ABC-ELISA法。用抗小鼠IFN-a单抗包被于酶标板上，标准品和样品中的IFN-a与单抗结合，加入生物素化的抗小鼠IFN-a，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin 查看更多
>
2021-08-15
v:* {behavior:url(#default#VML);}o:* {behavior:url(#default#VML);}w:* {behavior:url(#default#VML);}.shape {behavior:url(#default#VML);}st1:*{behavior:url(#ieoo 查看更多
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2018-12-26
st1:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable{mso-style-name:普通表格;mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-no 查看更多
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Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
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商品咨询
【求助】Elisa 竞争法标准曲线 免疫学讨论版123
livia19842009-02-03
Beckmann Kenko Gmbh | 获取完整的进口商历史记录 | 进口 ...123
txc6702009-04-01
【求助】竞争法ELISA的标准曲线 免疫学讨论版123
liuhua2018-01-17
大多数国产试剂盒生产厂家会提供对数坐标纸，可直接手工绘制曲线；如果酶标仪带有分析软件，可由软件直接拟合曲线，并自动计算出样本的浓度值；老式的酶标仪只能打印出OD值，需要实验员自己绘制曲线并计算，下面介绍2种绘制竞争法曲线的方法，希望对大家有帮助：一、在对数坐标纸上手工绘制曲线（Log-logit双对数标准曲线）1.实验做双孔，实验结果如下表所示。标准点浓度OD1OD2OD均结合率S002.2262.1922.209S10.11.7251.6791.70277.0%S20.41.3131.3381.325560.0%S31.60.9070.8340.870539.4%S46.40.5170.5170.51723.4%S525.60.2300.2440.23710.7%2）各标准点吸光值取均值。在表中OD均一列。3）将S1~S5的OD均值与S0的OD均相除，为标准点的百分结合率。4）在log-logit坐标纸上绘图。5）坐标纸上横轴从左至右第一个1-9表示为第一个10进位，第二个1-9表示为第二个10进位。第三个1-9表示为第三个10进位。也就是说，如果第一个1代表1ng/ml，则第二个1代表10ng/ml，第三个1代表100ng/ml。因为本次实验标准曲线范围是从0.1ng/ml到25.6ng/ml，则可以用第一个1代表0.1ng/ml，第二个1代表1ng/ml，第三个1代表10ng/ml。6）坐标纸纵轴为百分比。即各标准吸光值的百分结合率。7）曲线第一个数值点是（0.1，77.0%）。则在横轴左起点的1处向上至70~80之间，由70向上7个小格处。8）曲线第二个数值点是（0.4，60.0%）。则在横轴左起第一个4，向上至60的位置。9）曲线第三个数值点是（1.6，39.4%）。则在横轴左起第二个1至2之间，由第二个1向右6个小格。再向上至30~40之间，由30向上9个半小格处。10）曲线第四个数值点是（6.4，23.4%）。则在横轴左起第二个6至7之间，这里6与7之间分为5小格，则每个小格是0.2。所以由第二个6向右2个小格。再向上至20~30之间，由20向上约3个半小格处。11）曲线第四个数值点是（25.6，10.7%）。则在横轴左起第三个2至3之间，这里2与3之间分为10小格，则每个小格是1。所以由第三个2向右5个半小格。再向上至10~20之间，由10向上约半小格处。12)画一条通过各点的直线。要求尽可能多的点在线上，同时剩余的点均匀分布在直线的两边。13）样品也同样由吸光值计算百分结合率，再从纵轴上的相应结合率找到直线上的点，此点对应的横坐标浓度即为样品的浓度。无须换算。本数据来源于百度地图，最终结果以百度地图最新数据为准。
Elisa检测中样本OD值很低怎么办?公司动态上海恒远生物科技有限...123
hohoxian2016-07-18
说明微生物糖发酵实验的原理及结果分析。_123
hyf013462018-01-17
针对于HBeAb的中和抑制法
采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
竞争法ELISA的标准曲线 123
sonipi2021-07-21
【求助】竞争法ELISA试验为何同一样品在不同时间测得的OD值...123
txc6702021-07-25
竞争抑制ELISA标准曲线的拟合方法_抗原123
上山石头2021-07-27
ELISA实验结果常见问题及解决方案——标准曲线不佳123
vamtears2016-08-08
GM实验：试剂说明书上要求20分钟显色，10分钟的时候看一下，差不多就可以加终止液，但是我的标曲显色特别快，2分钟最低浓度蓝色就就深了，此时样本颜色还很浅很浅，我只能一块儿加终止液。所以，标曲显色过快，可能是什么原因？
（ps:第一列是标曲，后面是样本）
【求助】谁能告诉我最低检测限的计算方法 经验共享 分析测试...123
shile77722021-07-24
如题，我是菜鸟，网上看到有文章说用抑制率IC10作为最低检测限。不知是不是通用的做法，请各位战友不吝赐教
【求助】Elisa竞争法数据处理_问答详情_蚂蚁淘,【正品极速】生物...123
livia19842009-02-06
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