| ELISA 2.0 kit for measuring Felis domesticus allergen, Fel d 4. | ||||
| Antibodies: | 5F3/pAb | |||
| Standard Range: | 100-0.02ng/mL | |||
| Limit of Detection: | 0.08ng/mL | |||
| Background: | OD<0.08 at 450nm | |||
| Coefficient of Determination: | R-squared>0.98 | |||
| Contents: | Plate: Pre-coated with anti-Fel d 4 monoclonal antibody 5F3Vial 1: (white top) Fel d 4 allergen standardConcentration: 100ng/mlVial 2: (brown) Rabbit anti Fel d 4 antibodyVial 3: (blue top) Peroxidase-conjugated Goat Anti-RabbitBottle 1: Wash buffer, (10x concentrate)Bottle 2: Assay buffer, (10x concentrate)Bottle 3: TMB developing substrateBottle 4: Stop solution (0.5N sulfuric acid) | |||
| Storage: | Store at 2-8°C | |||
| Expiry: | 6 months from date of receipt | |||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized waterVolumetric measuring equipment (e.g. serological pipette, graduated cylinder)Clean containers for buffer and reagent preparationCalibrated single and multi-channel micropipettes and tipsVortex mixerPlate reader capable of reading absorbance at 450nmAnalysis software (recommended, but not required) | |||
| Notes: | The allergen standard is recommended for immunoassay calibrationpurposes only.A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. | |||
| Product Resources: | EPC-FD4-1 Certificate of Analysis Validation Performance Data | |||
For research and commercial use in vitro:not for human in vivo or therapeutic use. | ||||
| Certificate of Analysis | ||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated withmonoclonal antibody 5F3 and treated with stabilizingagent. Sealed in foil pouch with desiccant. | |||
| Monoclonal Antibody: | 5F3 | |||
| Immunogen: | Fel d 4 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to an epitope on cat Felis domesticus allergen, Fel d 4. | |||
| Purification: | Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Certificate of Analysis | ||||
| Detection Antibody: | Rabbit polyclonal antiserum | |||
| Immunogen: | Fel d 4 | |||
| Isotype: | Multiple | |||
| Specificity: | The antiserum contains IgG antibodies to cat Felis domesticus allergen, as well as IgG antibodies to other cat allergens. | |||
| Activity: | Titrated for use in ELISA for Fel d 4 allergen at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. | |||
| Certificate of Analysis | ||||
| Allergen Standard: | Recombinant Fel d 4 prepared in 1% BSA/50%glycerol/PBS, pH 7.4. | |||
| Concentration: | 100ng/mL (based on amino acid analysis) | |||
References: 1. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoorallergens and asthma: report of the Third International Workshop. J Allergy ClinImmunol 1997; 100(6 Pt 1):S2-24. 2. Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation,asthma, and a modified Th2 response in children exposed to cat allergen: apopulation-based cross-sectional study. Lancet 2001; 357(9258):752-756. 3. Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681 4. Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dustmite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. JAllergy Clin Immunol. 2007 Mar; 119(3):697-704. | ||||
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
