| Product Name | Streptavidin, 5 - TAMRA conjugated |
| Size | 1 mg |
| Catalog # | AS-60670 |
| US$ | $146 |
5-TAMRA-streptavidin conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function. 5-TAMRA is one of the most popular orange fluorophores used to label proteins. 5-TAMRA is the purified single isomer of 5-(and 6)-TAMRA that provides reproducibility for critical biological applications and avoids the inconsistency brought by minor positional difference between 5-TAMRA and 6-TAMRA. The fluorescence of 5-TAMRA can be observed at excitation/emission wavelength of 540 nm /575 nm. The 5-TAMRA-protein conjugates are very stable and suitable for immunofluorescent staining, fluorescence in situ hybridization, flow cytometry and other biological applications. | |
| Detailed Information | |
| Storage | refrigerated and desiccated |
| Molecular Weight | N/A |
| Spectral Properties | Abs/Em = 541/568 nm |
| Solvent System | water |
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采用抗-HBe抗体包被反应板,加入校准品及被测样本,同时加入定量HBeAg中和抗原,经过振荡孵育,洗板后再加入铕标记的抗-HBe,若标本中抗-HBe浓度高,HBeAg将被大量中和,使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液(β-NTA)将标记在抗体上的Eu3+解离到溶液中,Eu3+和增强液中的有效成分形成高荧光强度的螯合物,荧光强度和样本中的抗-HBe浓度成反比。
GM实验:试剂说明书上要求20分钟显色,10分钟的时候看一下,差不多就可以加终止液,但是我的标曲显色特别快,2分钟最低浓度蓝色就就深了,此时样本颜色还很浅很浅,我只能一块儿加终止液。所以,标曲显色过快,可能是什么原因?
(ps:第一列是标曲,后面是样本)

