Cellsome®-Natural PC extract catalog is composed of many liposomes made from phosphatidylcholine (PC) lipids that are made from natural lipids that are extracted from egg yolk, soybean, tissues and organs such as brain, heart and liver with and without cholesterol. Adding 30% cholesterol makes the liposomes serum stable and is necessary when the liposomes are used in the presence of serum.
Liposomes made from egg PC, contain both saturated and unsaturated lipids. These liposomes contain0.2% of the 14:0 lipid, 32.7% 16:0 lipid, 12.3% 18:0 lipid, 1.1% 16:1 lipid, 32% 18:1 lipid, 17.1% 18:2 lipid, 0.2% 20:2 lipid, and 0.3% 20:3 lipid, 2.7% 20:4 lipid and 0.4% 22:6 lipid.
Liposomes made from Brain PC contain both saturated and unsaturated lipids. They contain 0.3% 14:0 lipid, 30.6% 16:0 lipids, 16.5% 18:0 lipid, 0.9% 16:1 lipid, 33.3 % 18:1 lipid, 1.1% 18:2 lipid, 0.8% 20:1 lipid, 0.2% 20:3 lipid, 3.1% 20:4 lipid and 0.6% 22:6 lipid.
Liposomes made from Heart PC also contain 40% plasmalogen ether lipid. Ether lipids are more stable and do not get hydrolyzed. Heart PC also contains saturated and unsaturated lipids. It contains about 23% 16:0 lipid, 7% 18:0 lipid, 11% 18:1 lipid, 43% 18:2 lipid and 6% 20:4 lipid.
Liver PC also contains saturated and unsaturated lipids. Almost half of the lipids are saturated and the other half unsaturated. It is composed of 14% 16:0 lipid, 33% 18:0 lipid, 17% 18:1 lipid, 12% 18:2 lipid, 8% 20:3 lipid and 7% 20:4 lipid.
Soy PC also contains saturated and unsaturated lipids. Soy PC is mostly composed of unsaturated lipids and have a larger percentage of unsaturated PCs compare to egg, brain, heart and liver extracted PCs. Liposomes made from Soy PC contain 14.9% 16:0 lipid, 3.7% 18:0 lipid, 11.4% 18:1 lipid, 63% 18:2 lipid and 5.7% 18:3 lipid.
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
GM实验：试剂说明书上要求20分钟显色，10分钟的时候看一下，差不多就可以加终止液，但是我的标曲显色特别快，2分钟最低浓度蓝色就就深了，此时样本颜色还很浅很浅，我只能一块儿加终止液。所以，标曲显色过快，可能是什么原因？
（ps:第一列是标曲，后面是样本）
