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Norgen Biotek /microRNA Purification Kit (Cat. 21300)/25preps/21300
免费咨询热线
4000-520-616
- Extract high quality & purity miRNA in 30 minutes
- Two column kit- eliminate large RNA on first column and capture microRNA on a second column
- Elute microRNA in 25 µL ready for miRNA profiling
- Process a wide range of samples- cell, tissue, bacteria, bodily fluids, etc
- Buffer chemistry inactivates viruses including SARS-CoV-2 - Explore the Application Note
Related Services
NGS
RNA Isolation
Figure 1. High Quality of Isolated RNA with Better Size Exclusion. Small RNA was isolated from HeLa cells using Norgen"s microRNA Purification Kit (Lanes C and D) and a competitor"s kit (Lanes A and B). Ten microliters of the 50 µL purified small RNA were run on an 8% urea-agarose gel. Lane M is Norgen"s 100b RNA ladder. Note that Norgen"s kit is isolating only the small (<200 nt) RNA species, with no contaminating larger RNA.]">
microRNA Purification Kit Figure 1
Figure 2. Efficient Removal of Large RNA Species. Different RNA species were isolated from 106 HeLa cells, resolved on an Agilent® Lab-On-A-Chip and electropherograms were generated. Panel A contains all the RNA species present in 106 HeLa cells as isolated with Norgen"s Total RNA Purification Kit, and acts as a control. Panel B and C contain RNA that was isolated from 106 HeLa cells using Norgens microRNA Purification Kit. One microliter of the 50 µL purified RNA for each fraction was loaded. Panel B shows the large RNA species removed using the Large RNA Removal Columns, and no small RNA can be detected. Panel C shows the small RNA that is isolated using the microRNA Enrichment Columns, and shows that there is no contamination of the small RNA with any large RNA species above 200 nt. This demonstrates the effective separation of the small RNA from the large RNA species using Norgen"s microRNA Purification Kit.]">
microRNA Purification Kit Figure 2
Figure 3. Effective Small RNA Amplification by End-Point RT-PCR. Norgen"s microRNA Purification Kit isolates small RNA that could be amplified in RT-PCR using the poly (A) polymerase extension method. Small RNA was isolated from 1 million HeLa cells, and10µLof the 20 µL isolated small RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Seven microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was used in a 20 µL PCR reaction with primers against the human microRNAs (miR-21) and 5S rRNA. Panel A shows the amplification of the miR-21 transcript from small RNAs while Panel B shows the 5S rRNA amplification from small RNAs. Lane 1 in both panels shows the results when total RNAisolated from 1 million HeLa cells using Norgen"s Total RNA Purification Kit was used as a control. Lanes 2 and 3 contain the successful RT-PCR when the microRNA isolated using Norgen"s microRNA Purification Kit was used as the template, and Lanes 4 contain the non-template control. The RT-PCR was successful for both reactions using the microRNA as the template. All PCR products were resolved on a 1X TAE, 3% agarose gel using Norgen"s PCR Ranger asthe molecular weight ladder.]">
microRNA Purification Kit Figure 3
Figure 4. Better Recovery of miRNAs by Norgen"s microRNA Purification Kit. Norgen"s microRNA Purification Kit recovers microRNA more effectively than its competitors. Small RNA was isolated from 0.75 million HeLa cells using Norgen"s microRNA Purification Kit and a competitors"kits. Relative expression of (A) miR-21, (B) miR-19 and (C) 5S rRNA was determined by RT-qPCR of polyadenylated total RNA samples. RT-qPCR was performed according to Shi and Chiang (2005). Fifteen microliters of the 50 µL isolated RNA were polyadenylated in a 50 µL Poly-(A)-Polymerase reaction. Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. One microliter of the reverse transcription was then used in a 20 µL qPCR reaction with primers against the human microRNAs (miR-19 and miR-21) and house-keeping small RNA (5S rRNA). The resulting threshold cycle (Ct) values were summarized in the graph. Blue = Norgen"s microRNA Purification Kit; orange = Silica-basedCompetitor micro-RNA Kit; green = Silica-basedCompetitor Total RNA Kit; red = Phenol-based reagent protocol; yellow= No Template Control. Norgen"s microRNA Purification Kit recovered more miRNAs (lower Ct) than competitor"s microRNA-specific Kit.]">
microRNA Purification Kit Figure 4
microRNA Purification Kit
This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants and blood.
Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.
The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.
Background
These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...
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蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
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2018-12-26
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��试剂不同批号组分不得混用。10.如与英文说明书有异，以英文说明书为准。计算：以标准物的浓度为横坐标，OD值为纵坐标，在坐标纸上绘出标准曲线，根据样品的OD值由标准曲线查出相应� 查看更多
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠SCF单抗包被于酶标板上，标准品和样品中的SCF与单抗结合，加入生物素化的抗小鼠SCF，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin与生 查看更多
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠OrexinA单抗包被于酶标板上，标准品和样品中的OrexinA与单抗结合，加入生物素化的抗小鼠OrexinA，形成免疫复合物连接在板上，辣根过氧化物酶标记的Strepta 查看更多
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2018-12-26
采用双抗体夹心ABC-ELISA法。用抗小鼠PDGF单抗包被于酶标板上，标准品和样品中的PDGF与单抗结合，加入生物素化的抗小鼠PDGF，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin与 查看更多
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2018-12-26
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2021-09-08
采用双抗体夹心ABC-ELISA法。用抗小鼠LXA4/LipoxinA4单抗包被于酶标板上，标准品和样品中的LXA4与单抗结合，加入生物素化的抗小鼠LXA4，形成免疫复合物连接在板上，辣根过氧化物酶标记的Strept 查看更多
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2021-09-11
采用双抗体夹心ABC-ELISA法。用抗小鼠LPL单抗包被于酶标板上，标准品和样品中的LPL与单抗结合，加入生物素化的抗小鼠LPL，形成免疫复合物连接在板上，辣根过氧化物酶标记的Streptavidin与生 查看更多
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2021-08-09
本试剂盒应用双抗体夹心法测定标本中猪α干扰素(IFN-α)水平。用纯化的猪α干扰素(IFN-α)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入α干扰素(IFN-α)，再与HRP标记的IFN-α抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。 查看更多
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2019-04-15
蚂蚁淘生物2019年代理品牌目录 查看更多
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2018-12-26
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2019-03-12
GenDEPOT产品促销，品牌特约代理商 查看更多
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蚂蚁淘所售产品均为正品吗？
蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑；
Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
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商品咨询
国内外优质的Elisa试剂盒十大品牌_产品123
恋爱jiK硲32021-08-09
很多。回答里不好推荐。
ELISA试剂盒用哪个好一点 免疫学讨论版论坛123
MMM琪琪3HW52021-08-06
小鼠细胞因子用哪个牌子的试剂盒好一点，最好是国产的价格不要太高又有效果的，拜托啦
做血清、血浆、细胞的Elisa试剂盒可以测组织匀浆么?123
剑阁遗梦2021-08-10
人细胞角蛋白20(CK20)ELISA检测试剂盒哪家质量好_上海谷研实业...123
汾金融界凭2018-01-22
如何正确选择ELISA试剂盒123
闲逛7792021-08-08
第一步、明确检测何种蛋白
第二步、明确编码该蛋白的基因与其它哪些物种同源性最好
第三步、寻找检测这些物种蛋白的试剂盒
第四步、咨询商家ELISA是试剂盒使用的抗体类型:多抗还是单抗? 单抗是针对什么抗原决定簇的?
第五步、 做出自己的判断该买哪个试剂盒，或者实在没有可以找公司进行订制实验
第二步、明确编码该蛋白的基因与其它哪些物种同源性最好
第三步、寻找检测这些物种蛋白的试剂盒
第四步、咨询商家ELISA是试剂盒使用的抗体类型:多抗还是单抗? 单抗是针对什么抗原决定簇的?
第五步、 做出自己的判断该买哪个试剂盒，或者实在没有可以找公司进行订制实验
小鼠p53(p53)ELISA试剂盒品牌：TSZ,IBL,R&D等国内外知名品牌美...123
MMM琪琪3HW52021-07-25
做IL-2、IL-6、IFN-γ、TGF-β、sIgA和MPO这几个指标，感觉各种厂商差价有点大
国产的ELISA试剂盒哪个品牌比较好 123
凝帝系列978pHi2021-07-22
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ELISA试剂盒的作用有什么呢123
纯杰宗の62362018-01-22
ELISA检测试剂盒用途：
ELISA检测试剂盒是用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒 IgM抗体.
ELISA检测试剂盒是用于体外定性检测人血清或血浆中的抗人类戊型肝炎（HEV）病毒 IgM抗体.
请问这是蛇莓(空心泡)还是树莓? 123
一城暮雪2019-05-06
正确
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邹先京0532021-07-30
R&D Systems;Abnova;invitrogen;BD;Bender MedSystems;RayBiotech;Peprotech;CST;
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炎儿丶GAS2021-07-23
eBioscience、R&D的都有用，产品质量都是过硬的，没问题的，请放心选择！！！
国内和国外有哪些做elisa试剂盒的公司?要口碑好的,谢谢。123
liuhua2021-07-29
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