
High Ssensitivity | Reagents are optimized for greatest sensitivity of Firefly and Renilla luciferase signals |
---|---|
Low Background | Very low background luminescence. No subtraction is required from readings. |
Reproducibility | Reliable, linear results for a concentration range over several orders of magnitude. |
Compatibility | Compatible with Promega\\\\\\\\\\\\\\\"s Fluc and Rluc vectors. |
Versatility | Designed for assays with many different eukaryotic cell types (e.g. mammalian, lower invertebrates, etc.) using micro-plates or single-tube luminometers |
The LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kitprovides an efficient means of performing two reporter assays. In the LucyQ Duo-Luciferase(Firefly & Renilla) Assay, the activities of firefly (Photinus pyralis) andRenilla(Renilla reniformisor sea pansy) luciferases are measured sequentially from a single sample.
The firefly luciferase reporter is measured first by adding FLuc working solution to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and theRenillaluciferase reaction is initiated simultaneously by adding RLuc working solution to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors.
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从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式:
Biomiga(中国)
电话:0573-82651206
QQ:441931287
联系人:张小姐
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事
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