Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Source: Biotinylated recombinant SARS-CoV-2 Spike S1 protein (Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: QHD43416.1Predicted molecular mass: 77.9 kDa. Due to glycosylation, the biotinylated recombinant SARS-CoV-2 Spike S1 protein migrates to 110-120 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 1 EU per ug by the LAL method.Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein at 1 ug/ml (100 ul/well). Dose response curve for the recombinant human ACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA.Formulation: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
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它一般在医院、制药企业使用。
试剂盒使用示例: 试剂盒的产生正是为了使实验人员能够摆脱繁重的试剂配制及优化过程,所以试剂盒中一般配备有相应的使用说明书
从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式:
Biomiga(中国)
电话:0573-82651206
QQ:441931287
联系人:张小姐
对虾白斑综合症病毒(WSSV)核酸检测试剂盒(PCR-荧光探针法)
对虾白斑综合症病毒(WSSV)核酸检测试剂盒(恒温荧光法—一管式)
对虾桃拉综合症病毒(TSV)核酸检测试剂盒(恒温荧光法)
对虾桃拉综合症病毒(TSV)核酸检测试剂盒(PCR-荧光探针法)
对虾传染性皮下及造血组织坏死症病毒(IHHNV)核酸检测试剂盒(恒温荧光法)
对虾传染性皮下及造血组织坏死症病毒(IHHNV)核酸检测试剂盒(PCR-荧光探针法)
对虾肝胰腺细小病毒(HPV)核酸检测试剂盒(恒温荧光法)
对虾黄头病毒(YHV)核酸检测试剂盒(恒温荧光法)
对虾黄头病毒(YHV)核酸检测试剂盒(PCR-荧光探针法)
对虾杆状病毒(BP)核酸检测试剂盒(恒温荧光法)
对虾杆状病毒(BP)核酸检测试剂盒(PCR-荧光探针法)
对虾传染性肌肉坏死病毒(IMNV)核酸检测试剂盒(恒温荧光法)
对虾传染性肌肉坏死病毒(IMNV)核酸检测试剂盒(PCR-荧光探针法)
罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(恒温荧光法)
罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(PCR-荧光探针法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(恒温荧光法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(PCR-荧光探针法)
对虾偷死野田村病毒(CMNV)核酸检测试剂盒(恒温扩增法)
对虾偷死野田村病毒(CMNV)核酸检测试剂盒(PCR-荧光探针法)
过表达慢病毒
>10^8 PFU/ml
干扰慢病毒
>10^8 PFU/ml
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事

