Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Source: Biotinylated recombinant SARS-CoV-2 Spike S1 protein (Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: QHD43416.1Predicted molecular mass: 77.9 kDa. Due to glycosylation, the biotinylated recombinant SARS-CoV-2 Spike S1 protein migrates to 110-120 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 1 EU per ug by the LAL method.Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein at 1 ug/ml (100 ul/well). Dose response curve for the recombinant human ACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA.Formulation: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
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病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过？这个时候是用自备的针管还是试剂盒配的5毫升的针管啊？
从1～2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速，高效地从腺病毒感染的细胞培养液中分离纯化腺病毒，相比于传统CsCl超速离心的腺病毒病毒纯化方式（需要24个小时才能完成纯化），本试剂盒可以在1个小时内完成病毒纯化，操作简单，快速，纯化率高，纯化到的病毒颗粒可直接用于下游实验，如细胞和动物感染。
产品特点
?操作简单，快速，可以在1个小时内完成病毒纯化，不依赖于超速离心操作。
?纯化效率高：小量纯化，可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱，可以重复利用一次，用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃，其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式：
Biomiga(中国）
电话：0573-82651206
QQ:441931287
联系人：张小姐
试剂里面含有的是病毒的抗原，现在基本都是人工合成或提取的，跟病毒完全是两回事
如果用组织DNA提取试剂盒，提出来的就是组织细胞的DNA了
主要有： 1.磁珠法核酸提取试剂盒2.磁珠法提取DNA试剂盒3.DNA提取试剂盒（离心柱法）4.磁珠法RNA提取试剂盒5.RNA提取试剂盒（离心柱法）6.磁珠法植物基因组DNA提取试剂盒7.反转录试剂盒8.胶回收试剂盒9.PCR纯化试剂盒10.病毒核酸提取试剂盒
