GenCatch TM Plasmid DNA Mini-Prep Kit
| Catalog# | size | Price | Quantity | |
|---|---|---|---|---|
| 2160050 | 50 Mini Preps | $45.00 | ||
| 2160250 | 250 Mini Preps | $185.00 |
Description | GenCatch TM Plasmid DNAMini Preparation provides a simple, fast and cost-effectivemethod to purify plasmid DNA without phenol/chloroformextraction. It is based on binding of DNA to silica-basedmembranes in a chaotropic salt. An average yield of 1-20 ugplasmid DNA can be expected from 1-5 ml overnight bacterialculture. | ||||
Detailed Components | High quality spin columns, collection tubes together with buffers and protocols. | ||||
Features | Fast: Up to 24 samples can be readily prepared in 30 minutes with minimum efforts. Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenol extractions or ethanol precipitations required. Yield:4-20μg of DNA from 2ml of culture Quality DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing. | ||||
Technical Data |
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病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
过表达慢病毒
>10^8 PFU/ml
干扰慢病毒
>10^8 PFU/ml
主要有: 1.磁珠法核酸提取试剂盒2.磁珠法提取DNA试剂盒3.DNA提取试剂盒(离心柱法)4.磁珠法RNA提取试剂盒5.RNA提取试剂盒(离心柱法)6.磁珠法植物基因组DNA提取试剂盒7.反转录试剂盒8.胶回收试剂盒9.PCR纯化试剂盒10.病毒核酸提取试剂盒
它一般在医院、制药企业使用。
试剂盒使用示例: 试剂盒的产生正是为了使实验人员能够摆脱繁重的试剂配制及优化过程,所以试剂盒中一般配备有相应的使用说明书


Plasmid DNA ranging from 4 to 12 kb purified from E. coli DH5a with GenCatch Plasmid DNA Miniprep Kit and run on 1% agarose gel.
Restriction enzyme digestion of pBluescriptSK plasmid purified by GenCatchPlasmid DNA Miniprep Kit and analyzed on a 1.5% agarose gel.
Sequencing result of plasmid pBluescriptSK purified by GenCatchPlasmid DNA Miniprep Kit using ABI 3730xl sequencer.