- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody to human hepatitis virus C (HCV) non-structural protein NS-3
Purification:Protein G affinity purified
Product Type:Primary antibody
Target Protein:Human hepatitis C virus (HCV) non-structural protein NS-3
Immunogen:Purified recombinant chimeric HCV Polyprotein (555 amino acid residues)
Fusion Myeloma:Sp2/0-Ag14
Specificity:mAb 113 reacts with recombinant NS-3 (residues 1252-1477 on HCV polyprotein), synthetic NS-3 (residues 1378 -1458), and recombinant chimeric HCV polyprotein (60 kDa.)
Species Reactivity:Human hepatitis C virus, others not tested
Cross-Reactivity:No cross reaction can be seen with recombinant core protein C + envelop protein M(residues 1-142), synthetic core protein C (residues 1-61), and synthetic NS-4a protein (residures 1689-1735).
Host / Isotype:Mouse, IgG1 Kappa
Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.2
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Storage: Store at -20oC
Research Area:Virology
Background:
Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
Application:
ELISA:Reacts with HCV NS-3.
Western Blot:mAb clone 113, when used at concentration of 0.1-1μg/mL, will allow visualization of 100ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 protein. The mAb works on blots transferred from both reducing and non-reducing PAGE gel.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-HCV NS-3, 113 |
|---|---|
| Application | EIA, WB |
| Size | 0.1 mg |
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将来可能会出现基于免疫方法的试剂盒，不过因为H7N9是个新病毒，爆发至今不足一月，这么快时间还来不及生产抗体，所以目前的检测试剂盒只能是PCR检测试剂盒。
查到了：
4月7日，上海之江生物科技有限公司官方微博称，该公司成功研制禽流感H7N9(2013)核酸测定试剂盒(荧光PCR法)，是针对国内此次H7N9病毒最早研制成功的产品，也是国内目前唯一供应的成品化试剂盒。
