- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein
Purification:Protein G affinity purified
Product Type:Primary antibody, capture antibody in matched antibody pair
Target Protein:H. pylori cytotoxin associated gene A protein
Immunogen:A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.
Fusion Myeloma:Sp2/0-Ag14
Specificity:The mAb reacts with H. pylori CagA
Cross-Reactivity:Cross-reaction with other proteins has not been found.
Host / Isotype:Mouse, IgG2a Kappa
Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.0
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Storage: Store at -20oC
Research Area:Bacteriology
Background:
Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
Application:
ELISA: Clone 10E9 coated plate selectively reacts with cell lysate of a CagA containing H. pylori strain whenHRP conjugated anti-CagA clone 3C10 is used as detection antibody. In addition, clone 10E9 coated wells react with recombinant CagA antigen in combination withHRP conjugated anti-CagA mAb clone 5C6, clone 3C10, or clone 3C1.
Western Blot: This clone detects the 32 KD recombinant CagA antigen (immunogen).
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-HP CagA, 10E9, |
|---|---|
| Application | EIA, WB |
| Size | 0.1 mg |
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它一般在医院、制药企业使用。
试剂盒使用示例: 试剂盒的产生正是为了使实验人员能够摆脱繁重的试剂配制及优化过程,所以试剂盒中一般配备有相应的使用说明书
从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式:
Biomiga(中国)
电话:0573-82651206
QQ:441931287
联系人:张小姐
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对虾肝胰腺细小病毒(HPV)核酸检测试剂盒(恒温荧光法)
对虾黄头病毒(YHV)核酸检测试剂盒(恒温荧光法)
对虾黄头病毒(YHV)核酸检测试剂盒(PCR-荧光探针法)
对虾杆状病毒(BP)核酸检测试剂盒(恒温荧光法)
对虾杆状病毒(BP)核酸检测试剂盒(PCR-荧光探针法)
对虾传染性肌肉坏死病毒(IMNV)核酸检测试剂盒(恒温荧光法)
对虾传染性肌肉坏死病毒(IMNV)核酸检测试剂盒(PCR-荧光探针法)
罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(恒温荧光法)
罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(PCR-荧光探针法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(恒温荧光法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(PCR-荧光探针法)
对虾偷死野田村病毒(CMNV)核酸检测试剂盒(恒温扩增法)
对虾偷死野田村病毒(CMNV)核酸检测试剂盒(PCR-荧光探针法)
过表达慢病毒
>10^8 PFU/ml
干扰慢病毒
>10^8 PFU/ml
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事

