- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein
Purification:Protein G affinity purified
Product Type:Primary antibody
Target Protein:H. pylori cytotoxin associated gene A protein
Immunogen:A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.
Fusion Myeloma:Sp2/0-Ag14
Specificity:The mAb reacts with H. pylori CagA.
Cross-Reactivity:Cross-reaction with other proteins has not been found.
Host / Isotype:Mouse, IgG1 Kappa
Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.0
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Storage: Store at -20oC
Research Area:Bacteriology
Background:
Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
Application:
ELISA:Clone 5H10 can be used as capture antibody in sandwich ELISA to detect the recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 5H10 coated wells selectively react with cell lysate of CagA containing H. pylori strain when HRP conjugated anti-CagA clone 5C6 is used as detection antibody.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-HP CagA, 5H10 |
|---|---|
| Application | EIA |
| Size | 0.1 mg |
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病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过？这个时候是用自备的针管还是试剂盒配的5毫升的针管啊？
从1～2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速，高效地从腺病毒感染的细胞培养液中分离纯化腺病毒，相比于传统CsCl超速离心的腺病毒病毒纯化方式（需要24个小时才能完成纯化），本试剂盒可以在1个小时内完成病毒纯化，操作简单，快速，纯化率高，纯化到的病毒颗粒可直接用于下游实验，如细胞和动物感染。
产品特点
?操作简单，快速，可以在1个小时内完成病毒纯化，不依赖于超速离心操作。
?纯化效率高：小量纯化，可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱，可以重复利用一次，用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃，其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式：
Biomiga(中国）
电话：0573-82651206
QQ:441931287
联系人：张小姐
试剂里面含有的是病毒的抗原，现在基本都是人工合成或提取的，跟病毒完全是两回事
如果用组织DNA提取试剂盒，提出来的就是组织细胞的DNA了
主要有： 1.磁珠法核酸提取试剂盒2.磁珠法提取DNA试剂盒3.DNA提取试剂盒（离心柱法）4.磁珠法RNA提取试剂盒5.RNA提取试剂盒（离心柱法）6.磁珠法植物基因组DNA提取试剂盒7.反转录试剂盒8.胶回收试剂盒9.PCR纯化试剂盒10.病毒核酸提取试剂盒
