
Lactate dehydrogenase (LDH, LD) is a stable cytoplasmic enzyme present in most cells. During damage to (or rupture of) cell cytoplasmic membranes, LDH is releasedfrom cells and into the surrounding cell-culture supernatant. Quantitation of LDH in cell-culture supernatant is one methodby which investigatorsanalyze cell-death levels.
Lactate dehydrogenase (LDH, LD) is a stable cytoplasmic enzyme present in most cells. During damage to (or rupture of) cell cytoplasmic membranes, LDH is releasedfrom cells and into the surrounding cell-culture supernatant. Quantitation of LDH in cell-culture supernatant is one methodby which investigatorsanalyze cell-death levels.
The LDH Cytotoxicity Detection Kit is a simple yetsensitive, non-radioactive 96-well assayintended for the detection of lactate dehydrogenase (LDH, LD) that is releasedfrom damaged cells andinto the cell-culture supernatant. With this assay, LDH present in the surrounding cell-culturemedium participates in a coupled reaction that converts yellow tetrazolium salt (INT) into a red formazan product. The amount of LDH enzyme activity—measuredas a 490/492 nmabsorbancereading on amicroplate reader—directly correlateswith the number of damaged cells in culture. The entire assay procedure, whichincludes cell growth, chemical reaction and absorbancemeasurement, is performed in a single 96-well plate.
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过表达慢病毒
>10^8 PFU/ml
干扰慢病毒
>10^8 PFU/ml
对虾白斑综合症病毒(WSSV)核酸检测试剂盒(PCR-荧光探针法)
对虾白斑综合症病毒(WSSV)核酸检测试剂盒(恒温荧光法—一管式)
对虾桃拉综合症病毒(TSV)核酸检测试剂盒(恒温荧光法)
对虾桃拉综合症病毒(TSV)核酸检测试剂盒(PCR-荧光探针法)
对虾传染性皮下及造血组织坏死症病毒(IHHNV)核酸检测试剂盒(恒温荧光法)
对虾传染性皮下及造血组织坏死症病毒(IHHNV)核酸检测试剂盒(PCR-荧光探针法)
对虾肝胰腺细小病毒(HPV)核酸检测试剂盒(恒温荧光法)
对虾黄头病毒(YHV)核酸检测试剂盒(恒温荧光法)
对虾黄头病毒(YHV)核酸检测试剂盒(PCR-荧光探针法)
对虾杆状病毒(BP)核酸检测试剂盒(恒温荧光法)
对虾杆状病毒(BP)核酸检测试剂盒(PCR-荧光探针法)
对虾传染性肌肉坏死病毒(IMNV)核酸检测试剂盒(恒温荧光法)
对虾传染性肌肉坏死病毒(IMNV)核酸检测试剂盒(PCR-荧光探针法)
罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(恒温荧光法)
罗氏沼虾诺达病毒(MrNV)核酸检测试剂盒(PCR-荧光探针法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(恒温荧光法)
急性肝胰腺坏死病(AHPND/EMS)病原核酸检测试剂盒(PCR-荧光探针法)
对虾偷死野田村病毒(CMNV)核酸检测试剂盒(恒温扩增法)
对虾偷死野田村病毒(CMNV)核酸检测试剂盒(PCR-荧光探针法)
从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
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