Myelin is a substance rich in lipids and proteins that forms a layer, the myelin sheath, around only the axon of a neuron and acts as insulation. To detect the myelin sheath of peripheral nerves, the traditional method is to use osmic acid staining to obtain images for morphometrical assessment. However, osmic acid is extremely volatile and highly poisonous. It can fix corneas through vapor fixation which can lead to blindness. Inhalation of osmic acid can also lead to pulmonary edema and subsequent death, even at low exposure levels.Hito Nerve Myelin Sheath OptimStain™ Kit provides a safe and better alternative to osmic acid staining. The kit is non-toxic and easy to use with simplified procedures. It delivers stable and improved staining quality, with minimal overstains / background and artifacts when used properly.Hito Nerve Myelin Sheath OptimStain™ Kit has been tested on the frozen and paraffin transverse sections of peripheral nerves from several species of animals and proven to be sensitive for demonstrating the morphological details of myelin sheath. It is a simple solution for your research.
| Kit Contents (for 150 slides) | |
| Solution-1 | 240 ml |
| Solution-2 | 120 ml |
| Solution-3 | 120 ml |
| Solution-4 | 20 ml |
| Solution-5 | 125 ml |
| Solution-6 (Nissl Counterstain in Dropper Bottle) | 30 ml |
| Staining Jar | 2 |
| User Manual and MSDS | 1 |
Before using Hito Nerve Myelin Sheath OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat or MicrotomeDry ice, O.C.T. compound, isopentane, ethanol, xylene, double distilled or deionized waterPlastic/glass tubes, slides and coverslipsStaining jars for slides washResinous mounting medium Hito Nerve Myelin Sheath OptimStain™ Kit Manual and MSDS | |
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加尾法是采用加A酶先对mirna进行加尾,然后再用带oligodt的引物反转录,他的反转录引物是通用的,一次反转录可以获得所有miRNA的cdna,效率高。茎环法是采用特异性反转录引物序列+颈环结构作为反转录引物进行反转录的,一次反转录只能获得一种mirna的cdna,可能有几种一起反转录的,这个我不太清楚一起反转录的效果。然后茎环法的经典即ABI的探针法定量试剂盒。我用过茎环法反转录+染料法定量的,下游引物为通用引物,结果不太好,就现在用的这家的他采用的是双向引物都是特异性的,效果还可以。不知道我说的清楚不,希望能对你有帮助。
如果是用转录出的cDNA做那跟平时就一样了。不需要特别的试剂盒、


Hito Nerve Myelin Sheath OptimStain™ Kit Manual and MSDS