
Mycoplasma-ExS Spray is a reliable, all-around anti-microbial agent that is very safe and simple in its use. It is free of alcohols and harmful volatile organic compounds (VOCs) such as aldehydes. It is based on silver-polymer technology and provides a long-lasting, strong effect against mycoplasmas, bacteria, fungi and non-enveloped viruses. Mycoplasma-ExS is excellent for maintaining a clean, contamination-free work area.Mycoplasma-ExS Spray is highly effective against a broad spectrum of unwanted microbes commonly found in biological labs and has been validated according EN 13697. It completely eliminates reference microbes like Pseudomonas aeruginosa, Escherichia coli, Enteroccocus hirae, and Staphylococcus aureus (incubation time of 5 minutes at 22°C).The ready-to-use disinfectant does not irritate skin and it is not known to cause allergies. Moreover, it is neither carcinogenic (causing cancer) nor mutagenic (damaging genes). Mycoplasma-ExS Spray is excellent for cleaning and disinfection of hard laboratory surfaces and apparatuses, including laminar flow hoods, incubators, work benches, cell storage boxes, cryo-chambers, or instruments – making it ideal for laboratory everyday use.Mycoplasma-ExS Spray is non-corrosive to metal, does not damage plastic or rubber and is far less damaging to most surfaces than alcohol, bleach or hydrogen peroxide.
Aquaguard-1, a non-volatile water stabilizer/cleaner, is extremely effective at maintaining the water quality in CO2 incubators (tray). In addition, it does not cause damage to the stainless steel of the incubator or any plastic interior, and it does not cause any skin irritation when used as recommended. It comes as concentrated ready-to-use solution.
Aquaguard-2 is intended for maintenance of water quality in ordinary water baths. The active ingredient in Aquaguard-2 is highly effective to prevent growth of unwanted microorganisms such as bacteria and fungi. It is non-volatile, non-irritant and non-corrosive. Aquaguard-2 is stable at high temperatures (100°C) and does not produce foam in circulating water baths. It comes as concentrated ready-to-use solution.
Mycoplasma Disinfection Kit I not available in the US and Canada!
ebiomall.com






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请采纳。。。。
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品,而非原来clontech的产品,可单购。
3′-Full RACE Core Set Ver.2.0D314 20 次 1,800 元
5′-Full RACE Kit D315 RT反应10次 PCR反应50 次 3,980 元
takara的做得出来不,请各位高手指教,谢谢了!
步骤:1,RNA抽提(同RT)
2,逆转录,使用合成的oligo
3,p内参
4,使用根据已知序列设计的上游引物和提供下游引物做PCR,注意设计上游引物是退火温度与下游引物相差不大。
5,产物切胶回收,TA克隆测序
这个oligo和下游引物是我根据Takara和BD公司的manual自己设计的,经过我的使用效果很好。具体操作参照takara的3-racemanual。你先做3-race,了解熟悉下,成功后再做5-race。5-race要买试剂盒做,较为复杂!
我也想问一下。

