
- Overview
- Data/Specifications
- Literature/Support
- How To Use
Overview
Phenytoin is an anticonvulsant agent used to control various types of seizures in the brain or nervous system. It acts on voltage-gated sodium channels and stabilizes the threshold against hyperexcitability to reduce the electrical activity of brain cells.
Data/Specifications
Immunogen: Phenytoin-BTH
Host: Sheep
Format: Ig fraction
Ig Concentration: 9.63 mg/mL (UV abs @280 nm)
pH: 7.2
Preservative: sodium azide
Literature/Support
Phenytoin Polyclonal Antibody Insert (PDF)
How To Use
For use in enzyme immunoassays at a working dilution of 1.25 ug/mL
ebiomall.com






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请具体点,把联系方式和价位都说一下好吗?
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品,而非原来clontech的产品,可单购。
步骤:1,RNA抽提(同RT)
2,逆转录,使用合成的oligo
3,p内参
4,使用根据已知序列设计的上游引物和提供下游引物做PCR,注意设计上游引物是退火温度与下游引物相差不大。
5,产物切胶回收,TA克隆测序
这个oligo和下游引物是我根据Takara和BD公司的manual自己设计的,经过我的使用效果很好。具体操作参照takara的3-racemanual。你先做3-race,了解熟悉下,成功后再做5-race。5-race要买试剂盒做,较为复杂!

