Traditional 2D culture systems simply cannot support the scale-up required (>109cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions.
Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.
ebiomall.com
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请采纳。。。。
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品，而非原来clontech的产品，可单购。
3′-Full RACE Core Set Ver.2.0D314 20 次 1,800 元
5′-Full RACE Kit D315 RT反应10次 PCR反应50 次 3,980 元
takara的做得出来不，请各位高手指教，谢谢了！
步骤：1，RNA抽提（同RT）
2，逆转录，使用合成的oligo
3，p内参
4，使用根据已知序列设计的上游引物和提供下游引物做PCR，注意设计上游引物是退火温度与下游引物相差不大。
5，产物切胶回收，TA克隆测序
这个oligo和下游引物是我根据Takara和BD公司的manual自己设计的，经过我的使用效果很好。具体操作参照takara的3－racemanual。你先做3－race，了解熟悉下，成功后再做5－race。5－race要买试剂盒做，较为复杂！
我也想问一下。
