
The Diversify PCR Random Mutagenesis Kit employs a nontoxic method for mutagenizing sequences of up to 4.0 kb in length. The kit broadly distributes all possible mutations without creating hot spots. By allowing you to manipulate mutagenic conditions, the Diversify PCR Random Mutagenesis Kit provides optimal mutagenesis of sequences over an exceptionally broad size range. Select mutation rates from two to eight mutations per 1,000 bp simply by varying the amounts of two key reagents-manganese (Mn2+) and dGTP.
The Diversify PCR Random Mutagenesis Kit employs a nontoxic method for mutagenizing sequences of up to 4.0 kb in length. The kit broadly distributes all possible mutations without creating hot spots. By allowing you to manipulate mutagenic conditions, the Diversify PCR Random Mutagenesis Kit provides optimal mutagenesis of sequences over an exceptionally broad size range. Select mutation rates from two to eight mutations per 1,000 bp simply by varying the amounts of two key reagents—manganese (Mn2+) and dGTP (Cadwell and Joyce 1992; Leung, Chen, and Goeddel 1989).
The Diversify PCR Random Mutagenesis Kit contains our Titanium Taq PCR system to reliably amplify DNA fragments of up to 4.0 kb in length. This extended amplification range makes the Diversify kit ideal for mutating operons, plasmids, and sequences corresponding to large proteins, in addition to short DNA fragments. The kit also contains a rapid control reaction that allows the relative comparison of mutation rates in the control fragment in just two hours following PCR. In addition to the Titanium Taq polymerase mix, the Diversify PCR Random Mutagenesis Kit includes all of the reagents and buffers needed for mutagenesis.
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请具体点,把联系方式和价位都说一下好吗?
我也想问一下。
求采纳
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品,而非原来clontech的产品,可单购。
请采纳。。。。
做一次5-RACE的话,克隆10个以上基因应该是可以的。本数据来源于百度地图,最终结果以百度地图最新数据为准。

