
Numeroustechniqueshavebeendevelopedtoprepareimmunoliposomesbasedonthenucleophilicreactivityoffreeaminegroupsofproteinsorpeptides.OneofthemostpopularandcommonlyusedmethodsistocovalentlycouplefreecarboxylicgroupstoprimaryaminesthroughactivationofthecarboxylgroupswithEDC(1-ethyl-3-[3-dimethylaminopropyl]carbodiimide).EDC,whichisaso-calledzero-lengthcrosslinkingagent,reactswiththecarboxyltoformanaminereactiveintermediate(O-acylisourea).TheproducedO-acylisoureacanbeeasilydisplacedbynucleophilicattackfromtheprimaryaminogroupsinthereactionmixture.However,thisintermediateisunstableandhydrolyzedinaqueoussolutions.Inordertopreventtheintermediatehydrolysis,sulfo-NHS(N-hydroxysulfosuccinimide)isaddedtoEDCtoproduceasignificantlymorestableandmoresolubleactiveintermediate(NHSester).
Consequently,theimmunoliposomesarepreparedbyatwo-stepcouplingprocedure:first,activatingthefreecarboxylgroupofthelinkerlipidincorporatedintheliposomeswithEDCandsulfo-NHS,andthencovalentlyconjugatingtheantibodiestothelipidsthroughdisplacementofsulfo-NHSgroupsbyantibodyamines,asdepictedbelow.EDC/sulfo-NHScouplingreactionsarehighlyselectiveandhighlyefficient,andtheBIOLOGicalactivityoftheproteinorpeptideispreserved.

ImmunoFluor™-SuccinylisaPEGylatedproduct.Forotheraminereactive(PEGylatedandnon-PEGyalatedproducts)andalsoImmunoFluor™productssuitableforothertypesconjugationmethodsseehere.
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RACE是基于PCR技术基础上由已知的一段cDNA片段,通过往两端延伸扩增从而获得完整的3'端和5'端的方法
RACE技术的原理和操作方法:http://wenku.baidu.com/link?url=J-9lPSGmRLQriSfXhOqcYYFvJx7ktGIRMgU6k9vDQIywtdJ8deNg7Qi5t4CgtjxuhWUBvQO6_qsULiqUIFckEZty75jYNw5yz6rNqiIKCwm
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品,而非原来clontech的产品,可单购。