ABI荧光定量PCRA25742北京智杰方远现货900元促销!
品名 | 品牌 | 货号 | 规格 | 促销价 | ||
PowerUp™SYBR™GreenMasterMix | ABI | A25742 |
| 900 | ||
PowerUp™SYBR™GreenMasterMix | ABI | A25777 | 5x5mL | 询价 | ||
PowerUp™SYBR™GreenMasterMix | ABI | A257778 |
| 询价 |
规格
DetectionMethod: | SYBR |
ForUseWith(Equipment): | 7500FastSystem,7500System,7900HTSystem,QuantStudio™12kFlex,QuantStudio™3,QuantStudio™5,QuantStudio™6Flex,QuantStudio™7,StepOne™,StepOnePlus™,ViiA™7System |
ReactionSpeed: | FastorStandard |
PCRMethod: | qPCR |
SampleType(General): | DNA |
NumberofReactions: | 500reactions |
Polymerase: | Dual-Lock™TaqDNAPolymerase |
ProductLine: | PowerUP™,SYBR® |
ProductSize: | 5mL |
ShippingCondition: | WetIce |
内容及储存
The2xmixcontainsSYBRGreendye,Dual-LockTaqDNAPolymerase,dNTPswithdUTP/dTTPblend,heat-labileUDG,ROXpassivereferencedye,andoptimizedbuffercomponents.Contains1X5mLtube,sufficientfor50020-µLreactions(10µLMasterMixperreaction).Storeat2–8°C.
描述
PowerUp™SYBR®GreenMasterMixisapre-formulated,optimized,universal2Xmastermixforreal-timePCRworkflows.Coupledwithuser-suppliedprimersetsandtemplate,PowerUp™SYBR®GreenMasterMixisdesignedtoamplifiytargetsforaccurategeneexpressionanalysis.PowerUp™SYBR®GreenMasterMixfeaturesinclude:•Adualhot-startmechanismforexcellentspecificity•HighlyreproducibleCTsoverabroaddynamicrange•InclusionofUDGtohelppreventcarryovercontamination•Stabilityofpre-assembledreactionsforupto72hours•Compatibilitywithmostreal-timeqPCRinstrumentsExceptionalspecificitywithdualhot-startmechanismSpecificityisparamountinobtaininghigh-qualitydatainSYBR®GreenreactionsandcanbeenhancedthroughcontroloftheTaqDNApolymeraseatlowertemperaturesbeforestringentprimerbinding.PowerUp™SYBR®GreenMasterMixusestheDual-Lock™TaqDNApolymeraseenzymethatcombinestwouniquehot-startmechanismstohelpcontrolitsactivityandtopreventundesirableearlyactivityofthepolymeraseatlowtemperatures.Inanexperimentevaluating24differentprimersets,PowerUp™SYBR®GreenMasterMixgeneratedsinglemeltcurves100%ofthetime,consistentwithhighlyspecificamplification.TightreproducibilityinCTsoverawidedynamicrangePowerUp™SYBR®GreenMasterMixdemonstratesexcellentreproducibilityoverawidedynamicrangeforavarietyoftargetstested(seefigure).Inaddition,thismastermixprovidesefficientamplificationover6logsofsampleinput.UnlikecompetitormixesthatcanexhibitinhibitionathighcDNAinputsandlowcorrelationcoefficientsoveradynamicrangeexperiment,PowerUp™SYBR®GreenMasterMixefficiencyismaintainedoverthefulldynamicrangetohelpdelivermaximalconfidenceindataquality(seefigure).UDGincludedforcarryovercontaminationcontrolTheinclusionofuracil-DNAglycosylase(UDG)anddUTPinthePowerUp™SYBR®GreenMasterMixenablesthedegradationofanypreviouslyamplifiedPCRproducts,helpingpreventcontaminationofsubsequentqPCRreactionsandpossiblefalsepositives.Stabilityofpre-assembledreactionsThetightcontroloftheDual-Lock™TaqDNApolymeraseinPowerUp™SYBR®GreenMasterMixenablesreactionstobeassembledupto72hourspriortocyclingwithoutimpactingdata(seefigure).ThisconsistencyallowsuserstohaveconfidenceandflexibilityintheuseofPowerUP™SYBR®GreenMasterMixacrossnumerousworkflowscenarios1.BroadinstrumentcompatibilityPowerUp™SYBR®GreenMasterMixcanbeusedineitherstandardorfastcyclingmodeandiscompatiblewithallAppliedBiosystems®real-timePCRinstruments2.ItisalsocompatiblewiththeBio-RadIQ™5andCFX96™/CFX384™systems,RocheLightCycler®LC480,andStratagene®MX3005P®systems.Foroptimalresults,recommendedprimerconcentrationsare300–800nM.LearnmoreaboutPowerUp™SYBR®GreenMasterMix>
