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Bpsbioscience/PADI1/50151/10 µg
2024-05-03
Species: Human Host Species: Sf9 insect cells MW: 75 kDa Genbank #: NM_013358 Tag(s): C-terminal FLAG-tag a.a: 1-663(end) Description:Human PADI-1, also known as Peptidyl arginine deiminase, type I, (GenBank Accession No. NM_013358), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system. UniProt Q9ULC6 Synonym(s): PADI-1, Peptidyl arginine deiminase, type I, Pad Specific Activity: ≥35 pmol/min/μg Assay Conditions: Enzyme reaction was performed in a buffer(50 mM Tris, pH 7.4, 50 mM NaCl, 10mM CaCl2, and 2 mM DTT) containingPADI1 and 10 mM BAEE as a substratefor 25 min at 37°C. Amount of ammoniaproduced was measured and used forspecific activity calculation. Format: aqueous buffer solution Storage / Stability: >6 months at –70°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813–23. 2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384–93. 3. Guerrin M, et al. (2003). Biochem. J. 370 (Pt 1): 167–74. Warning(s): Avoid freeze/thaw cycles Scientific Category: Arginine Deiminase Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.
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