Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• High Activity

Xeno-free human IL-28B^HuXp (IFN-lambda 3) is expressed in human 293 cells with an apparent molecular mass of 24 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic human post-translational modification which contributes to stability in cell growth media and other applications. The purity is greater than 95%.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤1 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 24 kDa, monomer, non-glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent protection of the cytopathic effect on A549 cells (human lung adenocarcinoma epithelial cell line) that were challenged with encephalomyocarditis (EMC) virus.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).