Product Description:

• Endotoxin-free
• Animal-derived product free
• High Activity
• Authentic Glycosylation
• Lyophilized and Carrier Free (CF)

Xeno-free IL-2^HuXp is an O-glycosylated, four alpha -helix bundle cytokine. It is expressed by gamma T cells, CD4+ and CD8+ T cells, eosinophils, B cells, and dendritic cells. IL-2 has potent stimulatory activity for antigen-activated T cells. It is a monomeric glycoprotein with an apparent molecular mass of 15 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, which contributes to stability in cell growth media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 15 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).