QED Bioscience/CaMKII, phosphorylated /56454/1 Ea
Anti-phospho CaMKII Monoclonal AntibodyORDERING INFORMATIONCatalog No.: 56454 (clone 22B1) Size: 100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.BACKGROUND
CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron.
CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site.
Binding of calcium / calmodulin to its regulatory domain releases its auto- inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286.
Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.
SPECIFICATION SUMMARYAntigen: Synthetic peptide based on sequence from rat/mouse CaMKII alpha (aa 281-294), thiophosphorylated at residue Thr286 conjugated to a protein carrier.Host Species: MouseAntibody Class: IgG1 Preservatives: 0.09% sodium azide Other additives: 50% glycerolSPECIFICITYThis antibody recognizes the phosphorylated form of mouse and rat CaMKII. It does not react with non- phosphorylated CaMKII.
APPLICATIONSImmunoblotting: use at 1ug/ml. A band of
~50 kDa is detected. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain tissue extract.
DILUTION INSTRUCTIONSDilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITYThis antibody is stable for at least one (1) year at -20oC. Avoid repeated freezing and thawing.
For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.
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