ProZyme/Gly-X™ N-Glycan Rapid Release and Labeling with InstantPC™ Kit (24-ct) [GX24-IPC]/GX24-IPC/1
Description
The Gly-X™ N-Glycan Rapid Release and Labeling with InstantPC™ kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. After a simple clean-up step, the glycan samples are ready for analysis by UHPLC, LC-MS, MS/MS and other methods. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows.
Sample prep time: ~1 hour.
Other benefits include:
- Small molecule size for improved chromatographic peak resolution
- Flexible, high-throughput format: process 1 to 24 samples
- Optimized cleanup removes excess free dye, protein, and other interfering compounds
- Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 1 month
Contains:
- GX24-100 Gly-X Deglycosylation Module
- GX24-101 Gly-X InstantPC Labeling Module
- GX96-102 Gly-X InstantPC Clean-up Module
- Note: The 24-count kit includes a 96-count cleanup plate. This plate can be stored at room temperature for future use. Gly-X InstantPC digestion and labeling reagents (GX24-201PC) are available separately for use with existing cleanup plates.
Equipment and reagents provided by user:
- 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
- Thermocycler (Corning THERM-1001, 110V; THERM-1000, 230V)
- Note: Two GlykoPrep Heaters, WS0271, can be fitted with VWR 13259-260 Modular Heating Blocks
- Vacuum manifold (Millipore MSVMHTS00-HTS)
- Vacuum pump (Millipore WP6211560, 110 V; WP6122050, 220V; Welch WOB-L Pump 2522)
- Formic acid, MS-grade (Fisher A117-50 recommended)
- Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
Size: 1 kit (24-ct)
Product Code: GX24-IPC
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