Aldolase Antibody Properties Description Anti-ALDOLASE (GOAT) Antibody - 100-1141 Host Goat Target Species Rabbit Known Cross Reactivity rabbit, human Clonality Polyclonal Application ELISA : 1:5,000 - 1:20,000 Western Blot : 1:500 - 1:5,000 ImmunoPrecipitation: 1:100 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format Antiserum Label Unconjugated Concentration 90 mg/mL by Refractometry Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 2.0 mL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName ALDOA Relevant Links UniProtKB - P00883NCBI - P00883.2GeneID - 100009055

Aldolase Antibody Description

Synonyms goat anti-Aldolase Antibody, Fructose-bisphosphate aldolase A, Muscle-type aldolase Immunogen Aldolase [Rabbit Muscle] Immunogen Type Native Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note This product has been assayed against 1.0 µg of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.  Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate. Purity/Specifity This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.