ACE2 Antibody Properties Description Anti-ACE2 (RABBIT) Antibody - 600-401-X60 Host Rabbit Target Species Human Known Cross Reactivity Human Clonality Polyclonal Application ELISA : 1:5000-1:20,000 IF Microscopy : 20 µg/mL Western Blot : 0.5 - 2 µg/mL Immunohistochemistry: 2 µg/mL Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1 mg/mL by UV absorbance at 280 nm Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 Preservative 0.02% (w/v) Sodium Azide Stabilizer None GeneName ACE2 Relevant Links UniProtKB - Q9BYF1GeneID - 59272NCBI - NP_068576

ACE2 Antibody Description

Background Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. Synonyms ACE2 Antibody, ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH Immunogen Anti-ACE2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the internal region of human ACE2. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-ACE2 Antibody has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~90 kDa in size corresponding to ACE2 protein by Western Blotting in the appropriate cell lysate or extract. Purity/Specifity Anti-ACE2 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Anti-ACE2 has no cross response to ACE1. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.