ANGPTL3 Antibody Properties Description Anti-ANGPTL3 (RABBIT) Antibody - 600-401-Y15 Host Rabbit Target Species Human Known Cross Reactivity Human, mouse, rat Clonality Polyclonal Application ELISA : 1:10,000-1:20,000 IF Microscopy : 20 µg/mL Western Blot : 1 - 2 µg/mL Immunohistochemistry: 5 µg/mL Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1 mg/mL by UV absorbance at 280 nm Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 Preservative 0.02% (w/v) Sodium Azide Stabilizer None GeneName ANGPTL3 Relevant Links UniProtKB - Q9Y5C1GeneID - 27329NCBI - NP_055310

ANGPTL3 Antibody Description

Background The angiopoietin-related protein 3 (ANGPTL3) is a member of a family of secreted proteins that function in angiogenesis (1). ANGPTL3 is processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis (2,3). Recent experiments have shown that a deficiency of ANGPTL3 is associated with increased insulin sensitivity, lipoprotein lipase activity, and decreased serum free fatty acids, suggesting that ANGPTL3 may also play a role in modulating glucose metabolism (4). Synonyms ANGPTL3 Antibody, ANL3, ANG-5, FHBL2, ANGPT5, UNQ153/PRO179, Angiopoietin-related protein 3, Angiopoietin-5 Immunogen Anti-ANGPTL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the C-terminus of human ANGPTL3. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-ANGPTL3 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 54 kDa in Western Blots of specific cell lysates and tissues. Purity/Specifity Anti-ANGPTL3 antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. ANGPTL3 antibody is human, mouse and rat reactive. ANGPTL3 antibody is predicted not to cross-react with other ANGPTL family proteins. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.