Alcohol Dehydrogenase Antibody Biotin Conjugated Properties Description Anti-ALCOHOL DEHYDROGENASE (Yeast) (RABBIT) Antibody Biotin Conjugated- 200-4643-0100 Host Rabbit Target Species Yeast Known Cross Reactivity Saccharomyces cerevisiae (Bakers yeast) Clonality Polyclonal Application ELISA : 1:2,000 - 1:15,000 Western Blot : 1:500 - 1:5,000 ImmunoPrecipitation: 1:100 Other Dilution: User Optimized Physical State Lyophilized Shipping Condition Ambient Format IgG Label Biotin Concentration 1.0 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Reconstitution Volume 100 µL Reconstitution Buffer Restore with deionized water (or equivalent) Preservative 0.01% (w/v) Sodium Azide Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free GeneName ADH1, ADC1, YOL086C Relevant Links UniProtKB - P00330NCBI - 12643994GeneID - 2538902

Alcohol Dehydrogenase Antibody Biotin Conjugated Description

Background Alcohol Dehydrogenase is part of the zinc-containing alcohol dehydrogenase family. Anti-Alcohol Dehydrogenase catalyzes primary branched alcohols to convert to their aldehyde forms and shows some reactivity with secondary alcohols. Localized in the cytoplasm, the Anti-Alcohol Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cancer research. Synonyms rabbit anti-Alcohol Dehydrogenase Antibody biotin Conjugation, biotin conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC Immunogen Alcohol Dehydrogenase [Yeast] Immunogen Type Native Protein Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Application Note Anti-Alcohol Dehydrogenase has been assayed against 1.0 ug of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Purity/Specifity Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].  Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.