Ajuba Antibody Properties Description Anti-Ajuba (RABBIT) Antibody - 600-401-854 Host Rabbit Target Species Human Known Cross Reactivity human Clonality Polyclonal Application ELISA : 1:20,000 - 1:80,000 Western Blot : 1:500 - 1:2,500 ImmunoPrecipitation: 1:100 Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1.67 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName JUB Relevant Links NCBI - 14249622UniProtKB - Q96IF1GeneID - 84962

Ajuba Antibody Description

Background Human Ajuba (also called JUB protein and ajuba homolog isoform 1) is a LIM domain protein suggested to bind and regulate the activity of Aurora A.  Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes. Synonyms rabbit anti-Ajuba Antibody, Ajuba, JUB protein, ajuba homolog isoform 1 Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. Application Note This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to Ajuba by western blotting in the appropriate cell lysate or extract. Purity/Specifity This affinity purified antibody is directed against human Ajuba. The product was affinity purified from antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, mouse and chimpanzee based on 100% homology for the immunogen sequence.  Cross reactivity with Ajuba protein homologues from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.