APC1 phospho S377 Antibody Properties Description Anti-APC1 pS377 (RABBIT) Antibody - 600-401-877 Host Rabbit Target Species Human Known Cross Reactivity human Clonality Polyclonal Application ELISA : 1:2,000 - 1:10,000 Western Blot : 1:200 - 1:1,000 Immunohistochemistry: 5.0 µg/ml Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 0.21 mg/mL by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative 0.01% (w/v) Sodium Azide Stabilizer None GeneName ANAPC1 Relevant Links UniProtKB - Q9H1A4NCBI - 12056971GeneID - 64682

APC1 phospho S377 Antibody Description

Background APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle. Synonyms rabbit anti-APC1 pS377 Antibody, APC-1, APC 1, ANAPC1 antibody, Anaphase promoting complex subunit 1 antibody, Cyclosome subunit 1 antibody, MCPR antibody, Meiotic checkpoint regulator antibody Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 373-382 of Human Apc1 protein. Immunogen Type Peptide Post Translational Modification Phosphorylation Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract. Purity/Specifity This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross reactivity with APC1 homologues from other sources has not been determined.  Minimal reactivity is expected with the non-phosphorylated form of the protein. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.