AIFM3 Antibody Properties Description Anti-AIFM3 (RABBIT) Antibody - 600-401-X86 Host Rabbit Target Species Human Known Cross Reactivity Human, mouse, rat Clonality Polyclonal Application ELISA : 1:10,000-1:20,000 Western Blot : 1 - 2 µg/mL Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1 mg/mL by UV absorbance at 280 nm Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 Preservative 0.02% (w/v) Sodium Azide Stabilizer None GeneName AIFM3 Relevant Links UniProtKB - Q96NN9GeneID - 150209NCBI - Q96NN9

AIFM3 Antibody Description

Background Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist. Synonyms AIFM3 Antibody, AIFL, AIFL, Apoptosis-inducing factor 3, Apoptosis-inducing factor-like protein Immunogen Anti-AIFM3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human AIFM3. Immunogen Type Peptide Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-AIFM3 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 67 kDa in Western Blots of specific cell lysates and tissues. Purity/Specifity Anti-AIFM3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with AIFM3 from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.