Signosis/FewCell Stem Cell TF Activation Profiling Plate Array/FA-1103/1 Ea-蚂蚁淘商城

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Signosis/FewCell Stem Cell TF Activation Profiling Plate Array/FA-1103/1 Ea

2024-04-27

Description:

Stem cells are important cells characterized by their ability to self-renew or differentiate into many cell types, which is governed by intracellular signaling pathway and transcription regulation. Hence, transcription regulation plays a determining role in conferring cellular identity and function. The step-wise maturation of stem cells into terminally differentiated cell types requires the timely activation of a cascade of transcription programs governed by lineage-specifying transcription factors. Multiple transcription factors and target genes have been widely reported to associate with stem cell self-renewal and pluripotency including EGR1, OCT4, FOXD3, FOXO, Nanog, SOX2, SOX18, ETS, GLI, KLF4, MEF2, Myc, RNUX1, Pax6, TCF/LEF and GATA. However, stem cells are precious and limited.

Applicable Grid:

List of Applicable TFs:

123

78910

12AEGR1EtsEGR1

Ets

EGR1

Ets

EGR1EtsEGR1

Ets

EGR1

EtsBFOXD3GliFOXD3GliFOXD3GliFOXD3GliFOXD3GliFOXD3GliCFOXO1KLF4

FOXO1

KLF4FOXO1KLF4FOXO1KLF4

FOXO1

KLF4FOXO1KLF4DGATAMEF2GATAMEF2GATAMEF2GATAMEF2GATAMEF2GATAMEF2ENanogMycNanogMycNanogMycNanogMycNanogMycNanogMycFOCT4RNUX1OCT4RNUX1OCT4RNUX1OCT4RNUX1OCT4RNUX1OCT4RNUX1GSOX2PAX6SOX2PAX6SOX2PAX6SOX2PAX6SOX2PAX6

SOX2

PAX6HSOX18TCF/LEFSOX18TCF/LEFSOX18TCF/LEFSOX18TCF/LEFSOX18TCF/LEFSOX18TCF/LEF

Principle:

Signosis’ stem cell TF activation profiling plate array is used for monitoring the activation of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mix incubates with the cell lysate, individual probes will find its corresponding TF and form TF/probe complexes, which can be easily separated from free probes through simple spin column purification. The bound probes are detached from the complex and analyzed through hybridization with a plate; each well is specifically pre-coated with complementary sequences of the probes. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer.

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Data:

Stem cell TF activation profiling plate array analysis of TF activities in HeLa and Stem cell F9. The whole cell lysates were prepared and subjected to TF profiling assay.