Signosis/Mouse Cytokine cDNA Plate Array/AP-1141/1 Ea-蚂蚁淘商城

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Signosis/Mouse Cytokine cDNA Plate Array/AP-1141/1 Ea

2025-05-06

Description:

Cytokines are essential molecules that play crucial roles in many biological functions, including viral infection, inflammation, immunity, and hematopoiesis. Cytokines are produced by a variety of cell types in response to different stimuli. In addition, the expression of cytokine genes appears to be regulated by complex mechanism. Expression of one cytokine gene could be regulated by other cytokines. Dysregulation of cytokine gene expression may be caused by chromosomal alterations or by infection of viruses that induce activation or inactivation of the expression machinery. Therefore, expression profiling of cytokine genes could provide a useful approach to uncover the mechanism underlying their regulation.

Applicable Grid:

List of Applicable Genes

123456789101112A18S rRNAIL-13IL-418S rRNAIL-13IL-418S rRNAIL-13IL-418S rRNAIL-13IL-4BEotaxinIL-14IL-5EotaxinIL-14IL-5EotaxinIL-14IL-5EotaxinIL-14IL-5CGAPDHIL-15IL6GAPDHIL-15IL6GAPDHIL-15IL6GAPDHIL-15IL6DIFNbIL-16IL-7IFNbIL-16IL-7IFNbIL-16IL-7IFNbIL-16IL-7EIFNgIL-17TGFaIFNgIL-17TGFaIFNgIL-17TGFaIFNgIL-17TGFaFIL-10IL1ATGFbIL-10IL1ATGFbIL-10IL1ATGFbIL-10IL1ATGFbGIL11IL2TNFaIL11IL2TNFaIL11IL2TNFaIL11IL2TNFaHIL-12IL-3VEGFCIL-12IL-3VEGFCIL-12IL-3VEGFCIL-12IL-3VEGFC

Principle:

Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity. $\"\"$

Data:

$\"\"$ Mouse cytokine cDNA plate array analysis. NIH3T3 cells were treated with (red bars) or without (light blue) 10ng/ml TNF for 90 min. Cells were collected for profiling 23 genes with the cDNA plate array assay.