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CryoPur™ 使用说明翻译 - OriGen Biomedical,JC
2024-04-29
| 年限： | 1.5 years |
| 细胞形态： | 上皮样 |
| 数量： | 大量 |
| 运输方式： | 冻存运输 |
| 器官来源： | 乳腺 |
| 是否是肿瘤细胞： | 0 |
| 物种来源： | 小鼠 |
| 品系： | BALB/cRos |
| 生长状态： | 贴壁生长 |
| ATCC Number： | CRL-2116™ |
| 相关疾病： | 腺癌 |
| Designations: | JC |
| Depositors: | TM Chu |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent |
| Organism: | Mus musculus |
| Morphology: | epithelial |
| Source: | Organ: mammary gland Strain: BALB/cRos Disease: adenocarcinoma |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Tumorigenic: | Yes |
| Cytogenetic Analysis: | modal number = 40; range = 26 to 100 |
| Age: | 1.5 years |
| Gender: | female |
| Comments: | JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line. The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months. Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes. No type B or C virus particles were detected, although intracisternal A particles were observed occasionally. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Preservation: | Culture medium, 95%; DMSO, 5% |
| Doubling Time: | 12 to 16 hrs |
| References: | 22452: Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534 22679: Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384 |
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