产品简介 SiHa细胞应如何避免细胞污染，细胞污染的种类可分成细菌、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。SiHa细胞何时须更换培养基？视细胞生长密度而定，或遵照细胞株基本数据上之更换时间，按时更换培养基即可。 产品详细信息 SiHa细胞 细胞形态： 上皮样年限： grade II是否是肿瘤细胞： 1物种来源： 人器官来源： 宫颈ATCC Number： HTB-35™数量： 大量相关疾病： 鳞状细胞癌运输方式： 冻存运输生长状态： 贴壁生长SiHa细胞Designations: SiHaDepositors: Y ItoBiosafety Level: 2 [Cells contain human papilloma virus ]Shipped: frozenMedium Serum: See PropagationGrowth Properties: adherentOrganism: Homo sapiensMorphology: epithelial Source: Organ: cervixTumor Stage: grade IIDisease: squamous cell carcinomaPermits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SiHa细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Applications: transfection host (Roche Transfection Reagents)Tumorigenic: YesOncogene: p53 +; pRB +DNA Profile (STR): Amelogenin: XCSF1PO: 12D13S317: 11D16S539: 12D5S818: 9D7S820: 10THO1: 6,9TPOX: 8vWA: 14,17Cytogenetic Analysis: SiHa细胞modal number = 69; range = 51 to 72.This is a hypertriploid human cell line with the modal chromosome number of 71, occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2).Isoenzymes: AK-1, 1ES-D, 2G6PD, BGLO-I, 2Me-2, 1PGM1, 1PGM3, 1Age: 55 years *****Gender: femaleEthnicity: AsianComments: SiHa细胞This line was established from fragments of a primary tissue sample obtained after surgery from a Japanese patient.Electron microscopic observations revealed presence of typical desmosomes at the cell junctions and an abundance of tonofilaments in the cytoplasm.Mycoplasma contamination was detected and eliminated in 1975.The line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16, 1 to 2 copies per cell).Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle\'s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°CSubculturing: Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37�C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommendedMedium Renewal: 2 to 3 times per weekPreservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseRelated Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References: 22565: Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 302943022995: Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 299215323180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 299021723192: Friedl F, et al. Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proc. Soc. Exp. Biol. Med. 135: 543-545, 1970. PubMed: 552959823324: Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 164821829988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 918710532270: Olive PL, Banath JP. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res. 57: 5528-5533, 1997. PubMed: 9407963