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ubpbio/APPBP1/6xHis-UBA3/B1400/25 µg
2025-11-07
APPBP1/UBA3 is the heterodimeric E1 enzyme that conjugates Nedd8 to protein substrates. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to an E2 (Ubc12) to be finally attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin – RING E3 Ub ligases.
![](\"https://www.ebiomall.com/images/ubpbio/B1400_APPBP1_6XHis-UBA3.jpg\" "\\"B1400image\\"")
Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified APPBP1/6xHis-UBA3
Additional Information
| APPBP1/6xHis-UBA3 |
| APPBP1 (NAE1;HPP1; ula-1; A-116A10.1); UBA3 (UBE1C; hUBA3; MGC22384; DKFZp566J164) |
| B1400 |
| 25 µg |
| APPBP1 (60.2 kDa), UBA (51.8 kDa) |
| Human |
| Bacterial recombinant |
| 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| See tube label |
| ~95% by SDS-PAGE, see datasheet for gel image |
| Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Datasheet， MSDS |
| NM_003905 (APPBP1); NM_003968 (UBA3) |
| (Click image to enlarge) |
|
| Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified APPBP1/6xHis-UBA3 |
| Dry ice shipping |
| 1. Whitby FG, et al. (1998) J Biol Chem 273(52), 34983 – 34991.2. Osaka F, et al. (1998) Genes Dev 12(15), 2263 – 2268.3. Walden H, et al. (2003) Mol Cell 12(6), 1427 – 1437.4. Schulman BA, et al. (2009) Nat Rev Mol Biol 10(5), 319 – 331. |
Details
APPBP1/UBA3 is the heterodimeric E1 enzyme that conjugates Nedd8 to protein substrates. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to an E2 (Ubc12) to be finally attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin – RING E3 Ub ligases.Images:(Click image to enlarge)本文链接： https://www.ebiomall.cn/b459-ubpbio/info-1469707484.html
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