Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.

Additional Information

Product Name: Also Known As: Catalog No.: Size: Molecular Weight: Species: Source: Stock: Concentration: Quality Assurance: Storage: PDF Data Sheet: NCBI RefSeq: Image(s): Shipping Method: References:

K63-Ub3

K63-Ub3

D2201

50 µg

24.6 kDa

Human

Bacterial recombinant

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

See tube label

~95% by SDS-PAGE, see datasheet for gel image

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Datasheet， MSDS

N/A

N/A

Dry ice shipping

1. Hershko A, et al. (1980)Proc Natl Acad Sci USA 77(4), 1783 – 1786.2. Pickart CM, (1997) FASEB 11(13), 1055 – 1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479. 4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.

Details

Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.Images(Click image to enlarge)Coomassie-stained SDS-PAGELane 1: Molecular weight markersLane 2: 5 µg purified K63-Ub₂Lane 3: 5 µg purified K63-Ub₃Lane 4: 5 µg purified K63-Ub₄Lane 5: 5 µg purified K63-Ub₅Lane 6: 5 µg purified K63-Ub₆