货号：	4478359
英文名：	Total Exosome Isolation Reagent (from cell culture media)
规格：	50ml

Description

Total Exosome Isolation Reagent (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples.• Maximize recovery of intact exosomes for any type of downstream application• Easily isolate exosomes using our simple and reliable protocol• Avoid time-consuming ultra-centrifugation• Maximize flexibility—can be scaled up or down depending on the sample sizeIsolation of exosomes can be a tedious, non-specific, and difficult process. Total Exosome Isolation Reagent (from cell culture media) enables simple and reliable concentration of intact exosomes from cell media samples with a protocol that is scalable depending on your sample size.By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the cell media sample, and the solution is incubated overnight at 2°C to 8°C. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 60 min. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA and protein can then be purified using the Total Exosome RNA and Protein Isolation Kit, or the intact exosomes can be used for biological studies of their pathways, functions, and trafficking. For immunoprecipitation of exosomal proteins, use Exosome Immunoprecipitation (Protein A) or Exosome Immunoprecipitation (Protein G).To isolate intact exosomes from blood serum samples, we recommend Total Exosome Isolation Reagent (from serum).‹ View less View more › For Research Use Only. Not for use in diagnostic procedures.

Figures

Analysis of the exosomal miRNA levels by quantitative RT-PCR.

NanoSight analysis of the exosomes recovered from HeLa cell media with Total Exosome Isolation reagent.

Western blot analysis for the presence of exosomal marker protein CD63 in samples recovered with Total Exosome Isolation reagent.

Specifications

Reagent Type:Final Product:Product Size:Sample Type (Specific):Shipping Condition:

Exosome Isolation Reagent

Exosomes

50 mL

Cell Culture Media

Room Temperature

Contents & storage

Store at 2 to 8 °C.北京明阳科华生物科技有限公司联系人：吴经理联系电话：15011527870/13701245989座机/传真：010-59919166QQ客服1：204057474QQ客服2：1170535434Email：mingyangkehua@126.com地址：北京市朝阳区汤立路216号东方郁金香C座8层邮编100012   温馨提示：不可用于临床治疗。