常规说明 The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As 存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity. 发表研究结果有使用 ab45074？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab45074 被引用在 5 文献中. Soler-Arnedo P et al. Polarly Localized EccE1 Is Required for ESX-1 Function and Stabilization of ESX-1 Membrane Proteins in Mycobacterium tuberculosis. J Bacteriol 202:N/A (2020).PubMed: 31843799 Lai LY et al. Role of the Mycobacterium marinum ESX-1 Secretion System in Sliding Motility and Biofilm Formation. Front Microbiol 9:1160 (2018).PubMed: 29899738 Lou Y et al. EspC forms a filamentous structure in the cell envelope of Mycobacterium tuberculosis and impacts ESX-1 secretion. Mol Microbiol 103:26-38 (2017).PubMed: 27859904 Sharma G et al. The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG methylation and histone H3/H4 binding. Nucleic Acids Res 43:3922-37 (2015). HumanPubMed: 25824946 Ramsdell TL et al. Linked domain architectures allow for specialization of function in the FtsK/SpoIIIE ATPases of ESX secretion systems. J Mol Biol 427:1119-32 (2015).PubMed: 24979678 Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) Concentration: 5% Temperature: 4 C Sample Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate) Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5 g/mL Temperature: 22 C Thank you for your interest in Abcam. 1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding sequences of these have unfortunately not been determined, except for ab26246 (residues EQQWNFAGIEAAA at positions 3-15). So if you are working with protein fragments (or epitopes), we cannot guarantee that these antibodies will work for you, as the binding sites might not be present and therefore the antibody might not bind. I could ask the lab whether the epitopes have been mapped and whether the antibodies would bind your fragments, if you could provide some details to your peptides. 2) We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service: United States Pocono Rabbit Farm and Laboratory (PRFL) PO Box 240 Canadensis, PA 18325 Tel: 800-622-6381 - Toll Free in the United Statesand Canada Tel: 570-595-7108 - Worldwide Fax: 570-595-9277 Website: www.prfal.com Maine Biotechnology Services, Inc. 1037R Forest Ave. Portland Maine 04103 Tel: 800-925-9476 or 207-797-5454 Fax: 207-797-5595 Website: www.mainebiotechnology.com Cocalico Biologicals, Inc. PO Box 265 449 Stevens Road Reamstown, PA 17567-0265 Tel: 717-336-1990 Fax: 717-336-1993 Website: www.cocalicobiologicals.com United Kingdom Charles River Laboratories International, Inc. Tel: +44 (0) 1843 823575 Website: www.criver.com I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions. Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com