Full length protein corresponding to Mouse AHA1 aa 1-350.
Database link: Q8BK64

WB: A431 cells, A549 cells, HCT116 cells, Hela cells, HEK293 cells, HepG2 cells, HL-60 cells, HUVEC cells, Jurkat cells, MCF7 cells, PC3 cells, T98G cells, rat brainICC/IF: HeLa cells.
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存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The Abpromise guarantee

Abpromise™承诺保证使用ab83036于以下的经测试应用

\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

Activator of 90 kDa heat shock protein ATPase homolog 1 antibody Activator of heat shock 90kDa protein ATPase homolog 1 antibody AHA 1 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA1 Polyclonal Antibody (ab83036). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody (ab83036) at 1:60 for 12 hours at 4 C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Endoplasmic reticulum. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA1 Antibody. (C) Composite. Heat Shocked at 42 C for 1h.

All lanes : Anti-AHA1 antibody (ab83036)

Lane 1 : Cell lysates prepared from A431 cells
Lane 2 : Cell lysates prepared from A549 cells
Lane 3 : Cell lysates prepared from HCT116 cells
Lane 4 : Cell lysate prepared from Hela cells
Lane 5 : Cell lysate prepared from HEK293 cells
Lane 6 : Cell lysates prepared from HepG2 cells
Lane 7 : Cell lysates prepared from HL-60 cells
Lane 8 : Cell lysates prepared from HUVEC cells
Lane 9 : Cell lysates prepared from Jurkat cells
Lane 10 : Cell lysates prepared from MCF7 cells
Lane 11 : Cell lysates prepared from PC3 cells
Lane 12 : Cell lysates prepared from T98G cells
Lane 13 : Lysate prepared from rat brain

Lysates/proteins at 10 µg per lane.

Predicted band size: 38 kDa

发表研究结果有使用 ab83036？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab83036 被引用在 3 文献中.

Shelton LB et al. Hsp90 activator Aha1 drives production of pathological tau aggregates. Proc Natl Acad Sci U S A 114:9707-9712 (2017).PubMed: 28827321 Ghosh S et al. Endoplasmic Reticulum Resident Heat Shock Protein 90 (Hsp90) Isoform Glucose-Regulated Protein 94 (Grp94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport. J Biol Chem N/A:N/A (2016).PubMed: 26872972 Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014).PubMed: 24465380
The first 3 antibodies were raised against the full length mouse or human protein, respectively.
The sequence homology with these proteins and zebrafish is 60% whereas the residues 1-31 have a homology of 100%. However, the epitope has not been mapped and we do not know where the antibody will bind the protein.
Only ab64998 was raised against an internal peptide sequence. I am in touch with the lab to check what region had been selected and to check homology.
We usually say that a homology of 85% or higher has a good chance of cross reactivity. With 60% homology I am not sure. If the epitope happens to be in the N-terminal region however, the likelihood of binding to the zebrafish protein would be very high, though.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far.
(https://www.abcam.com/Hsp90-antibody-16F1-ab13494.html) - it would be guaranteed for this species as well as the listed tested applications.
For Aha1, we do not have an antibody tested in this species. But if you please could send me the protein sequence for zebrafish, I\'d be happy to check homology and to see whether any of the Aha1 antibodies can be used for this species.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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