Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EPR4646] to FLI1 - BSA and Azide free Suitable for: WB, IHC-P Reacts with: Mouse, Human, Recombinant fragment Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. U937, Jurkat, Raw264.7 and HL-60 cell lysates; Human Ewing sarcoma tissue; FLI1 recombinant protein 常规说明 ab215987 is the carrier-free version of ab133485.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar is a trademark of Fluidigm Canada Inc.This product is a recombinant monoclonal antibody, which offers several advantages including:- High batch-to-batch consistency and reproducibility- Improved sensitivity and specificity- Long-term security of supply- Animal-free productionFor more information see here.Our RabMAb technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb patents. The Abpromise guarantee Abpromise™承诺保证使用ab215987于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 WBUse at an assay dependent concentration. Predicted molecular weight: 51 kDa. IHC-PUse at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. Sequence-specific transcriptional activator. Recognizes the DNA sequence 5\'-C[CA]GGAAGT-3\'. 疾病相关 Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. 序列相似性 Belongs to the ETS family.Contains 1 ETS DNA-binding domain.Contains 1 PNT (pointed) domain. FLI1_HUMAN antibody Friend leukemia integration 1 (FLI1) transcription factor antibody Friend leukemia integration 1 transcription factor antibody Friend leukemia virus integration 1 antibody Proto-oncogene Fli-1 antibody Sarcoma breakpoint region 2 (EWSR2) antibody SIC 1 antibody SIC1 antibody Transcription factor ERGB antibody Viral integration region FLI1 antibody Viral integration region FLI1, mouse, homolog of antibody see all Immunohistochemical staining of paraffin embedded human lung with purified ab133485 at a working dilution of 1 in 700. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133485). Immunohistochemical staining of paraffin embedded human lung with unpurified ab133485 at a working dilution of 1 in 140. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133485). Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labelled with unpurified ab133485 at 1/250 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133485).Perform heat mediated antigen retrieval before commencing with IHC staining protocol. To download a Certificate of Compliance, please enter your Lot number below:Lot number 发表研究结果有使用 ab215987？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab215987 尚未被引用在任何文献中。 There are currently no Customer reviews or Questions for ab215987.Please use the links above to contact us or submit feedback about this product.Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com