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Adipogen/Ubiquitin aldehyde (human) (rec.) (HA)/AG-40T-0512-C050/50 µg

  
  2025-09-20
  
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Ub-H
Protein
E. coli
Human ubiquitin (Accession Nr. P0CG47) tagged to aldehyde and fused to a N-terminal HA-tag.
Human
Lyophilized from a solution in MES.
Use: Potent and specific inhibitor of most deubiquitinating enzymes (DUBs) such as ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). Prevents the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-ubiquitin chain accumulation. The HA-tag allows for detection and purification of deubiquitinating enzyme activity. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 2-5µM for in vitro assays to inhibit deubiquitinating enzymes.
Manufactured by Boston Biochem
BLUE ICE
+4°C
-20°C
Aliquot to avoid freeze/thaw cycles.
Stable for at least 1 year after receipt when stored at -20°C.
No
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Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting.

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