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Adipogen/STAM-1 (human) (rec.)/AG-40T-0358-C050/50 µg

  
  2025-09-20
  
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Signal Transducing Adapter Molecule 1
Protein
E. coli
Full-length human STAM-1 (Accession Nr. Q92783).
Human
Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 2mM DTT, 2mM EDTA .
Use: Has no intrinsic deubiquitinase activity but is useful in stimulating the in vitro activity of the JAMM-class deubiquitinase AMSH. Reaction conditions for stimulation of AMSH will need to be optimized for each specific application. Binds AMSH protein with Kd = ~7µM. In in vitro assays containing 200nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM. Half-maximal increase occurred at 0.4µM STAM.
Manufactured by Boston Biochem
DRY ICE
-20°C
-80°C
Aliquot to avoid freeze/thaw cycles.
Stable for at least 1 year after receipt when stored at -80°C.
No
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Signal Transducing Adaptor Molecule-1 (STAM-1) is a member the STAM family of adaptor molecules. This ubiquitously expressed protein is 540 amino acids (aa) in length with a predicted molecular weight of 59.18 kDa. Human STAM-1 shares 91% and 92% aa sequence identity with the rat and mouse orthologs, respectively. It contains multiple domains including a VHS domain (aa 16-143) and a ubiquitin-interacting motif (aa 171-190), both of which bind ubiquitinated proteins, a SH3 domain (aa 210-269) that associates with the zinc metalloprotease STAMBP and an ITAM domain (aa 370-387), which contains a Hrs-binding site and interacts with Janus kinases (Jaks). STAM-1 is involved in downstream signaling stimulated by cytokines and growth factors. It is tyrosine phosphorylated by Jaks in response to a variety of cytokines including IL-2, IL-3, IL-4, IL-7, GM-CSF, EGF and PDGF, and has been shown to induce DNA synthesis and MYC expression. Additionally, STAM-1 has been shown to regulate CXCR4 signaling and appears to be important for T cell development and survival. Besides its role in signal transduction, STAM-1 may also be important for the regulation of endocytic membrane trafficking. It associates with Hrs to form the ESCRT-0 complex, which binds to ubiquitinated membrane proteins on early endosomes and directs them to the ESCRT-1 complex for lysosomal degradation. STAM-1 has also been shown to interact with coat protein II complexes and function in trafficking vesicles from the endoplasmic reticulum to the golgi apparatus.

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